Document 0725
 DOCN  M95A0725
 TI    In vivo cellular tropism of human T-lymphotropic virus type II is not
       restricted to CD8+ cells.
 DT    9510
 AU    Lal RB; Owen SM; Rudolph DL; Dawson C; Prince H; Retrovirus Diseases
       Branch, Centers for Disease Control and; Prevention, Atlanta, Georgia
       30333, USA.
 SO    Virology. 1995 Jul 10;210(2):441-7. Unique Identifier : AIDSLINE
       MED/95343556
 AB    We have examined the in vivo and in vitro susceptibility of lymphocyte
       subpopulations to human T-lymphotropic virus type II (HTLV-II) to
       determine the cellular tropism for this virus. Monoclonal antibodies to
       T-cell subsets were used to separate highly purified CD4+ and CD8+ cells
       from peripheral blood lymphocytes of 35 individuals previously shown to
       be infected with HTLV-II. The purified T-cell subsets were analyzed for
       HTLV-II provirus (pol and tax gene sequences) by polymerase chain
       reaction (PCR) and cultured to determine virus expression by p24gag
       antigen detection. On the basis of PCR amplification in the pol and tax
       gene regions, both CD8+ subsets (89 to 91%) and CD4+ subsets (54 to 80%)
       from most infected subjects demonstrated HTLV-II provirus, irrespective
       of the viral genotype. Analysis of cultured lymphocytes demonstrated a
       higher spontaneous lymphocyte proliferation (17,986 +/- 4675 cpm) and
       p24gag antigen production (median 115 pg/ml; range 14-1360 pg/ml) in
       CD8+ cells compared to CD4+ cells (2333 +/- 826 cpm; p24gag antigen; 9
       pg/ml; 2-250 pg/ml), suggesting a higher proviral load in CD8 cells.
       Limiting cell-dilution PCR analysis indicated that the CD8+ subset
       carried a higher HTLV-II provirus burden than the CD4+ subset. In vitro
       infection of purified CD4+ and CD8+ lymphocytes with irradiated HTLV-II
       cell lines resulted in productive infection of both subsets. Cell
       sorting and PCR analysis of lymphocyte subsets from 4 HTLV-II-infected
       subjects further demonstrated that in addition to CD4+ and CD8+ subsets,
       both CD45RO+ and CD45RO- and non-T-cells (CD14, CD16, and CD19) carried
       HTLV-II provirus. Taken together, these data suggest that HTLV-II
       possesses a broad tropism for peripheral blood mononuclear cells.
 DE    Antibodies, Monoclonal  Antigens, CD/ANALYSIS  Antigens,
       Differentiation, B-Lymphocyte  Base Sequence  Cell Division  Cell
       Separation  Cells, Cultured  CD4-Positive
       T-Lymphocytes/CYTOLOGY/*VIROLOGY  CD8-Positive
       T-Lymphocytes/CYTOLOGY/*VIROLOGY  DNA, Viral/ANALYSIS  Genes,
       pol/GENETICS  Genes, pX/GENETICS  Human  HTLV-II/GENETICS/*PHYSIOLOGY
       Leukocytes, Mononuclear/VIROLOGY  Molecular Sequence Data  Proviruses
       Retroviridae Proteins, Oncogenic/BIOSYNTHESIS  T-Lymphocyte
       Subsets/CYTOLOGY/*VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).