Document 0727
 DOCN  M95A0727
 TI    Evaluation of an autologous red cell agglutination test, VetRED FIV, for
       the presence of FIV antibody in cats.
 DT    9510
 AU    del Fierro GM; Bundesen P; Martin S; Jones S; Beetson S; Robinson WF;
       School of Veterinary Studies, Murdoch University, Australia.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):93-101. Unique Identifier :
       AIDSLINE MED/95343535
 AB    A commercially available whole blood agglutination test, VetRED FIV,
       used for the detection of antibodies to feline immunodeficiency virus
       (FIV), was evaluated. The test is based on the use of a synthetic
       peptide conjugated to a non-agglutinating anti-feline red blood cell
       monoclonal antibody. The amino acid sequence of the synthetic peptide
       was derived from the predicted sequence of the transmembrane protein of
       FIV. The sensitivity and specificity of VetRED FIV was 100% when 34
       known FIV-positive and 15 known FIV-negative cats were tested. These
       cats were part of studies on experimentally induced FIV infection, with
       their FIV status confirmed by virus isolation. Further, VetRED FIV was
       compared with another commercially available test for FIV antibody,
       PetChek in a field trial on 548 feline blood samples received by a
       diagnostic laboratory. Of the test results 94.2% (516/548) were in
       agreement: 112 were positive by VetRED FIV and PetChek; 404 were
       negative by both tests and 32 were discordant. These 5.8% discordant
       samples producing VetRED FIV-positive/PetChek-negative or VetRED
       FIV-negative/PetChek-positive were further assessed by Western blot
       assay. In the field trial, the sensitivity and specificity of VetRED FIV
       was 97% and 97%, respectively, comparable to the 98% sensitivity and 99%
       specificity for PetChek. The results from the trial also confirm the
       relatively high overall prevalence of FIV in Australian cats
       predominantly among mature male cats in the 9-12 year age group. Given
       the simplicity of the VetRED FIV procedure, it is concluded that VetRED
       FIV is a useful addition to the available commercial tests for FIV
       infection.
 DE    Animal  Antibodies, Viral/*BLOOD  Blotting, Western/VETERINARY  Cats
       Comparative Study  Enzyme-Linked Immunosorbent Assay/VETERINARY
       Erythrocytes/*IMMUNOLOGY  Evaluation Studies  False Negative Reactions
       Feline Acquired Immunodeficiency Syndrome/*DIAGNOSIS  Female
       Hemagglutination/IMMUNOLOGY  Hemagglutination Tests/*VETERINARY
       Immunodeficiency Virus, Feline/*IMMUNOLOGY  Male  Peptide
       Fragments/IMMUNOLOGY  Predictive Value of Tests  Reproducibility of
       Results  Sensitivity and Specificity  Support, Non-U.S. Gov't  Viral
       Envelope Proteins/IMMUNOLOGY  JOURNAL ARTICLE

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