Document 0728
 DOCN  M95A0728
 TI    Serological diagnosis of feline immunodeficiency virus infection using
       recombinant transmembrane glycoprotein.
 DT    9510
 AU    Calzolari M; Young E; Cox D; Davis D; Lutz H; Department of Internal
       Veterinary Medicine, University of Zurich,; Switzerland.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):83-92. Unique Identifier :
       AIDSLINE MED/95343534
 AB    We developed an antibody detection enzyme-linked immunosorbent assay
       (ELISA) using recombinant surface (SU), transmembrane (TM) and capsid
       (CA) antigens of feline immunodeficiency virus (FIV) expressed in
       Escherichia coli. The three antigens were tested with sera collected
       from experimentally infected cats in order to follow the course of
       seroconversion and of the antibody levels throughout the infection. An
       early and marked increase of TM antibodies was observed. Antibodies to
       TM were demonstrated at high levels throughout the observation period.
       The immune response to SU and to CA was less pronounced and in some cats
       the level of antibodies to SU and CA tended to decline 6 months after
       infection. In addition, 413 FIV negative and positive cat sera were
       tested in order to define for each antigen the diagnostic sensitivity,
       specificity and efficiency. TM showed the highest diagnostic sensitivity
       (98%) while its specificity was 97%. Its diagnostic efficiency of 97%
       was better than that of SU and CA and exceeded that of tests utilizing
       conventionally grown and gradient purified FIV. Therefore, recombinant
       TM can be considered a very important antigen for FIV ELISA testing. An
       interesting perspective is offered in the combination of TM with other
       recombinant antigens in a dot assay form.
 DE    Animal  Antibodies, Viral/*BLOOD  Blotting, Western/VETERINARY
       Capsid/*IMMUNOLOGY  Cats  Enzyme-Linked Immunosorbent Assay/VETERINARY
       Feline Acquired Immunodeficiency Syndrome/*DIAGNOSIS/IMMUNOLOGY
       Immunodeficiency Virus, Feline/*IMMUNOLOGY  Molecular Weight
       Recombinant Proteins/IMMUNOLOGY  Sensitivity and Specificity  Specific
       Pathogen-Free Organisms  Support, Non-U.S. Gov't  Viral Envelope
       Proteins/*IMMUNOLOGY  JOURNAL ARTICLE

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