Document 0729
 DOCN  M95A0729
 TI    The feline lymphoid cell line MBM and its use for feline
       immunodeficiency virus isolation and quantitation.
 DT    9510
 AU    Matteucci D; Mazzetti P; Baldinotti F; Zaccaro L; Bendinelli M;
       Department of Biomedicine, University of Pisa, Italy.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):71-82. Unique Identifier :
       AIDSLINE MED/95343533
 AB    We report on the development of a feline T lymphoblastoid cell line
       obtained from the peripheral blood mononuclear cells (PBMC) of a
       specific pathogen free cat and designated MBM. The cells are pan-T+,
       CD4- and CD8- and remained interleukin-2-dependent and concanavalin
       A-dependent throughout the period of observation. MBM cells have proved
       at least as sensitive as fresh blasts to infection with cell-free stocks
       of three feline immunodeficiency virus (FIV) isolates. Upon infection,
       they exhibit a lytic cytopathic effect. Repeated attempts to establish a
       chronic infection have failed. Using a limiting cell dilution method, it
       has been shown that MBM cells may be more sensitive than fresh blasts as
       substrate for isolating FIV from the PBMC of infected cats. These
       studies have also shown that considerable individual variations exist in
       the virus loads present in the PBMC of naturally infected cats, and that
       load size does not appear to correlate with cat age, clinical status,
       CD4/CD8 ratio and titer of serum neutralizing antibody.
 DE    Animal  Cats  Cell Line  Cells, Cultured  Feline Acquired
       Immunodeficiency Syndrome/*VIROLOGY  Flow Cytometry/VETERINARY
       Immunodeficiency Virus, Feline/*ISOLATION & PURIF/PHYSIOLOGY
       Immunophenotyping/VETERINARY  Karyotyping/VETERINARY  Leukocytes,
       Mononuclear/VIROLOGY  Lymphoid Tissue/CYTOLOGY  Specific Pathogen-Free
       Organisms  Support, Non-U.S. Gov't  T-Lymphocytes/IMMUNOLOGY/*VIROLOGY
       Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).