Document 0730
 DOCN  M95A0730
 TI    A determinant of feline immunodeficiency virus involved in Crandell
       feline kidney cell tropism.
 DT    9510
 AU    Siebelink KH; Karlas JA; Rimmelzwaan GF; Osterhaus AD; Bosch ML;
       Laboratory of Immunobiology, National Institute of Public Health; and
       Environmental Protection, Antonie van Leeuwenhoeklaan,; Bilthoven, The
       Netherlands.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):61-9. Unique Identifier :
       AIDSLINE MED/95343532
 AB    Viral progeny of the molecular clone 19k1 of feline immunodeficiency
       virus (FIV) can infect feline T-cells but not Crandell feline kidney
       (CrFK) cells. In contrast, the biological isolate FIV-AM6c, which was
       CrFK adapted by co-cultivation of FIV-AM6 infected thymocytes with CrFK
       cells, can infect both thymocytes and CrFK cells. The envelope gene of
       FIV-AM6c was amplified by polymerase chain reaction using DNA from
       infected CrFK cells, and subsequently cloned and sequenced. To map viral
       determinants of CrFK cell tropism, chimeric viruses with a 19k1
       background containing envelope gene fragments of FIV-AM6c were
       constructed. CrFK cells were transfected with DNA of these chimeric
       clones and co-cultivated with thymocytes. After 3 days the CrFK cells
       and the thymocytes were cultured separately. FIV antigen could be
       detected in most of the thymocyte cultures within 14 days and in one of
       the CrFK cultures after 52 days. The resulting virus from this CrFK
       culture can infect both CrFK cells and thymocytes. The results of this
       study indicate that the envelope region contains determinants of CrFK
       tropism. The delay in replication indicates that also determinants other
       than those identified here are involved in CrFK cell tropism. More
       chimeric clones are being studied at present to map these determinants.
 DE    Amino Acid Sequence  Animal  Antigenic Determinants/*ANALYSIS  Antigens,
       Viral/ANALYSIS  Cats  Cell Line  Cells, Cultured  Chimera  DNA,
       Viral/GENETICS  Enzyme-Linked Immunosorbent Assay/VETERINARY
       Immunodeficiency Virus, Feline/*PHYSIOLOGY  Kidney/CYTOLOGY/*VIROLOGY
       Molecular Sequence Data  Polymerase Chain Reaction/VETERINARY  Specific
       Pathogen-Free Organisms  Support, Non-U.S. Gov't  T-Lymphocytes/VIROLOGY
       Transfection  Tropism/*PHYSIOLOGY  Viral Envelope
       Proteins/GENETICS/IMMUNOLOGY  Virus Replication/*PHYSIOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).