Document 0731
 DOCN  M95A0731
 TI    Two different mutations in the envelope protein of feline
       immunodeficiency virus allow the virus to escape from neutralization by
       feline serum antibodies.
 DT    9510
 AU    Siebelink KH; Bosch ML; Rimmelzwaan GF; Meloen RH; Osterhaus AD;
       Laboratory of Immunobiology, National Institute of Public Health; and
       Environmental Protection, Antonie van Leeuwenhoeklaan,; Bilthoven, The
       Netherlands.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):51-9. Unique Identifier :
       AIDSLINE MED/95343531
 AB    Viral progeny of two molecular clones of feline immunodeficiency virus
       (FIV), 19k1 and 19k32, were tested in a virus neutralization assay. In
       this assay the infection of thymocytes with FIV19k1 was neutralized by
       serum S1422, derived from an SPF cat 22 weeks after infection with
       FIV19k1. We previously reported that a point mutation at position 560 in
       hypervariable region-5 (HV-5) of 19k1 confers resistance to virus
       neutralization (Siebelink et al., 1993, J. Virol. 67:2202-2208). Viral
       progeny of the other molecular clone, FIV19k32, which differs in the
       envelope protein in only six amino acids from 19k1, was not neutralized.
       In order to map sites involved in virus neutralization we constructed
       chimeric clones by reciprocal exchange of 19k1 and 19k32 envelope gene
       fragments. Reciprocal exchange of a 1662 bp fragment, encoding almost
       the whole surface protein, which differs in five amino acids between
       these two clones, resulted in exchange of the phenotype. Amino acids of
       the envelope protein of 19k1 and 19k32, in which these clones differ,
       were substituted by point mutation. We demonstrated that one of these
       mutations, a substitution of leucine to serine at position 483 in HV-4,
       also conferred resistance of 19k1 to neutralization by serum S1422.
 DE    Amino Acid Sequence  Animal  Antibodies, Viral/*IMMUNOLOGY  Antigenic
       Determinants/IMMUNOLOGY  Antigens, Viral/IMMUNOLOGY  Base Sequence  Cats
       Chimera  Feline Acquired Immunodeficiency Syndrome/*IMMUNOLOGY  Female
       Immunodeficiency Virus, Feline/*GENETICS/IMMUNOLOGY  Molecular Sequence
       Data  Molecular Weight  Mutagenesis, Site-Directed  Neutralization
       Tests/VETERINARY  *Point Mutation  Specific Pathogen-Free Organisms
       Support, Non-U.S. Gov't  Viral Envelope Proteins/*GENETICS/IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).