Document 0732
 DOCN  M95A0732
 TI    Tumor necrosis factor-alpha responses are depressed and interleukin-6
       responses unaltered in feline immunodeficiency virus infected cats.
 DT    9510
 AU    Ma J; Kennedy-Stoskopf S; Sellon R; Tonkonogy S; Hawkins EC; Tompkins
       MB; Tompkins WA; Department of Microbiology, Pathology, College of
       Veterinary; Medicine, North Carolina State University, Raleigh 27606,
       USA.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):35-50. Unique Identifier :
       AIDSLINE MED/95343530
 AB    Feline immunodeficiency virus (FIV), a lentivirus similar to HIV, causes
       an acquired immunodeficiency syndrome in cats. Similar to human
       immunodeficiency virus (HIV), the pathogenesis of FIV is associated with
       dysregulation of the cytokine network. While alterations in tumor
       necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) expression
       have been reported in HIV-infected patients, changes attributable to HIV
       and those caused by cofactors such as secondary infections cannot always
       be readily distinguished. This study evaluated the effect of FIV
       infection on TNF-alpha and IL-6 production in cats not exposed to other
       potential cofactors such as secondary infections. TNF-alpha and IL-6
       activities were evaluated in bronchoalveolar lavage (BAL) cells from
       FIV-infected and uninfected specific pathogen free (SPF) cats.
       Supernatants from lipopolysaccharide (LPS)-stimulated BAL cells from
       uninfected SPF cats had high levels of TNF-alpha and IL-6 activity,
       while stimulated BAL cell supernatants from FIV-infected SPF cats had
       significantly lower levels of TNF-alpha but unaltered IL-6 activity.
       Similarly, Con A/phorbol myristate acetate (PMA) stimulated non-adherent
       (NA-) peripheral blood mononuclear cells (PBMC) from FIV infected cats
       synthesized less TNF-alpha than similarly treated NA-PBMC from
       uninfected cats. Feline immunodeficiency virus could be recovered from
       the culture supernatants of BAL cells from infected cats by
       co-cultivation with susceptible lymphocytes. In situ hybridization
       identified FIV mRNA in a small fraction of alveolar macrophages in the
       BAL cell cultures.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Animal  Bronchoalveolar Lavage Fluid/CYTOLOGY  Cats  Cells, Cultured
       CD4-Positive T-Lymphocytes  Feline Acquired Immunodeficiency
       Syndrome/*METABOLISM  Immunodeficiency Virus,
       Feline/GENETICS/*IMMUNOLOGY/ISOLATION &  PURIF  In Situ
       Hybridization/VETERINARY  Interleukin-6/*BIOSYNTHESIS  Leukocytes,
       Mononuclear/*METABOLISM  Lipopolysaccharides/PHARMACOLOGY  Lymphocyte
       Transformation/DRUG EFFECTS  Macrophages, Alveolar/VIROLOGY
       Mitogens/PHARMACOLOGY  RNA, Messenger/ANALYSIS  RNA, Viral/ANALYSIS
       Specific Pathogen-Free Organisms  Support, U.S. Gov't, P.H.S.  Tumor
       Necrosis Factor/*BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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