Document 0734
 DOCN  M95A0734
 TI    Panleukopenia-like syndrome of FeLV caused by co-infection with FeLV and
       feline panleukopenia virus.
 DT    9510
 AU    Lutz H; Castelli I; Ehrensperger F; Pospischil A; Rosskopf M; Siegl G;
       Grob M; Martinod S; Department of Veterinary Medicine, University of
       Zurich,; Switzerland.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):21-33. Unique Identifier :
       AIDSLINE MED/95343528
 AB    To study the effect of interferon on feline leukemia virus (FeLV)
       infection, 30 specific pathogen free (SPF) cats were infected with the
       apathogenic FeLV A Glasgow. Unexpectedly, between 5 and 8 weeks after
       FeLV infection, all 19 cats with persistent FeLV infection but not the
       FeLV-negative cats died from a panleukopenia-like syndrome. No feline
       panleukopenia virus (FPLV) antigen was found in feces by latex
       agglutination, enzyme-linked immunosorbent assay (ELISA) or
       immunoelectron microscopy. No enteropathogenic bacteria were found.
       Histopathology revealed changes resembling those of FPLV infection such
       as destruction of crypts and pancytopenia of bone marrow. Neither
       clinical signs nor seroconversion to FPLV could be induced by
       transmitting intestinal extracts to two SPF cats. However, FPLV antigen
       was demonstrated by immunofluorescence assay in intestinal cryostat
       sections of diseased animals. FPLV could also be demonstrated in
       intestinal extracts by immunoelectron microscopy, by latex agglutination
       and ELISA after anti-FPLV antibodies were removed from immune-complexed
       FPLV by ultracentrifugation over a CsCl gradient at pH 2.0. From these
       experiments it was concluded that the panleukopenia-like syndrome of
       FeLV may not be caused by FeLV alone but at least in some cases by
       co-infection with FeLV and FPLV. In addition, some form of 'cooperation'
       between FeLV and FPLV must be postulated because neither virus alone
       induced symptoms.
 DE    Animal  Antibodies, Viral/BLOOD  Antigens, Viral/ANALYSIS  Cats
       Centrifugation, Density Gradient/VETERINARY  Enzyme-Linked Immunosorbent
       Assay/VETERINARY  Feces/MICROBIOLOGY  Feline Acquired Immunodeficiency
       Syndrome/*COMPLICATIONS/  IMMUNOLOGY/PATHOLOGY  Feline Infectious
       Enteritis/*ETIOLOGY/IMMUNOLOGY/PATHOLOGY/  TRANSMISSION  Fluorescent
       Antibody Technique/VETERINARY  Interferon Alfa, Recombinant/PHARMACOLOGY
       Latex Fixation Tests/VETERINARY  Leukemia Virus,
       Feline/*IMMUNOLOGY/ISOLATION & PURIF/  ULTRASTRUCTURE  Lymphoid
       Tissue/PATHOLOGY/VIROLOGY  Microscopy, Immunoelectron/VETERINARY
       Parvovirus, Feline/*IMMUNOLOGY/ISOLATION & PURIF/ULTRASTRUCTURE
       Specific Pathogen-Free Organisms  Support, Non-U.S. Gov't  Syndrome
       Viremia/IMMUNOLOGY/VETERINARY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).