Document 0736
 DOCN  M95A0736
 TI    Dehydroepiandrosterone inhibits replication of feline immunodeficiency
       virus in chronically infected cells.
 DT    9510
 AU    Bradley WG; Kraus LA; Good RA; Day NK; All Children's Hospital,
       Laboratory of Retrovirology, St.; Petersburg, FL 33701, USA.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):159-68. Unique Identifier :
       AIDSLINE MED/95343525
 AB    Dehydroepiandrosterone (DHEA) is a steroid hormone produced by the
       adrenal cortex that serves as an intermediary in sex steroid synthesis.
       DHEA is produced in abundance by humans and most other warm-blooded
       animals. Based upon previous reports demonstrating the antiviral and
       immunostimulatory activities of DHEA and DHEA-sulfate (DHEAS) we sought
       to determine whether introduction of these compounds would affect
       replication of feline immunodeficiency virus (FIV) in chronically
       infected cells. When cell number, cell viability, cellular DNA
       synthesis, and levels of FIV reverse-transcriptase (RT) were measured in
       cell cultures treated with various dilutions of DHEA or DHEAS it was
       found that the production of FIV RT was inhibited by DHEA at levels
       where cellular viability and DNA synthesis were not affected. At the
       concentrations tested DHEAS did not inhibit FIV replication or impact on
       cellular viability or proliferation.
 DE    Animal  Cats  Cell Count  Cell Division  Cell Line  Cell Survival
       Cells, Cultured  DNA, Viral/BIOSYNTHESIS  Immunodeficiency Virus,
       Feline/DRUG EFFECTS/*PHYSIOLOGY  Prasterone/*PHARMACOLOGY  Reverse
       Transcriptase/DRUG EFFECTS  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  T-Lymphocytes/*VIROLOGY  Virus Replication/*DRUG EFFECTS
       JOURNAL ARTICLE

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