Document 0738
 DOCN  M95A0738
 TI    Vaccination against feline immunodeficiency virus using fixed infected
       cells.
 DT    9510
 AU    Verschoor EJ; van Vliet AL; Egberink HF; Hesselink W; van Alphen WE;
       Joosten I; Boog CJ; Horzinek MC; de Ronde A; Department of Infectious
       Diseases and Immunology, Veterinary; Faculty, University Utrecht, The
       Netherlands.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):139-49. Unique Identifier :
       AIDSLINE MED/95343523
 AB    Crandell feline kidney cells and feline thymocytes, either feline
       immunodeficiency virus (FIV) infected or uninfected, were fixed with
       paraformaldehyde and used to vaccinate cats. The cells were mixed with a
       30:70 water/mineral oil emulsion containing 250 micrograms ml-1
       N-acetyl-D-glucosaminyl-beta-(1-4)-N-acetyl-muramyl-L-alanyl-D-is-
       oglutam ine. Eighteen specific pathogen-free cats were vaccinated three
       times with 3-week intervals and challenged 21 days after the final boost
       with a low dose of the homologous FIV-UT113 strain. Eight out of ten
       cats that had received FIV-infected cell vaccines developed significant
       anti-FIV antibody titres to the envelope and core antigens. Neutralizing
       antibodies were detectable at the moment of challenge in the sera of
       these animals. Within 5 weeks after challenge 15 out of 18 cats became
       viraemic. Three animals, two that had been vaccinated with FIV-infected
       thymocytes and did not develop antibody, and one that had received an
       uninfected thymocyte preparation, remained uninfected for 6 months. Upon
       rechallenge of the three animals, two again resisted infection; these
       cats had been immunized with the infected and the uninfected thymocyte
       preparations, respectively.
 DE    Animal  Antibodies, Viral/BIOSYNTHESIS  Base Sequence  Cats  Cell Line
       Cells, Cultured  DNA Primers/CHEMISTRY  Feline Acquired Immunodeficiency
       Syndrome/IMMUNOLOGY/*PREVENTION  & CONTROL  Immunodeficiency Virus,
       Feline/GROWTH & DEVELOPMENT/*IMMUNOLOGY  Kidney/CYTOLOGY/VIROLOGY  Major
       Histocompatibility Complex/IMMUNOLOGY  Molecular Sequence Data
       Polymerase Chain Reaction/VETERINARY  Specific Pathogen-Free Organisms
       Support, Non-U.S. Gov't  T-Lymphocytes/VIROLOGY  Tissue Fixation
       Vaccination/*VETERINARY  Viral Structural Proteins/GENETICS/IMMUNOLOGY
       *Viral Vaccines/IMMUNOLOGY  Viremia/IMMUNOLOGY/PREVENTION &
       CONTROL/VETERINARY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).