Document 0739
 DOCN  M95A0739
 TI    Renal involvement in feline immunodeficiency virus infection: p24
       antigen detection, virus isolation and PCR analysis.
 DT    9510
 AU    Poli A; Abramo F; Matteucci D; Baldinotti F; Pistello M; Lombardi S;
       Barsotti P; Bendinelli M; Department of Animal Pathology, University of
       Pisa, Italy.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):13-20. Unique Identifier :
       AIDSLINE MED/95343522
 AB    Renal alterations characterized morphologically by glomerular and
       tubulo-interstitial lesions and clinically by a heavy proteinuria and
       sometimes by renal failure are frequent in feline immunodeficiency virus
       (FIV) infected cats. To investigate the possible role of local FIV
       replication in the genesis of this renal damage, renal tissues of 15
       consecutive naturally infected and five non-infected cats were examined
       for traces of the virus by immunohistochemistry, using a monoclonal
       anti-p24 antibody in a streptavidin-biotin peroxidase labeled system,
       cultivation and polymerase chain reaction (PCR). Tubular epithelial
       cells as well as scattered interstitial inflammatory and glomerular
       cells were positive for p24 antigen in 13 cats. Viral isolation was
       successful in seven cats, and FIV gag DNA and RNA sequences were
       detected in 14 and five cats, respectively. Control cats were constantly
       negative. Although not conclusive, these results suggest that a direct
       role of FIV in the induction of the renal damage observed in infected
       animals is possible.
 DE    Animal  Antigens, Viral/ANALYSIS  Cat Diseases/*VIROLOGY  Cats  DNA,
       Viral/ANALYSIS  Enzyme-Linked Immunosorbent Assay/VETERINARY  Feline
       Acquired Immunodeficiency Syndrome/*VIROLOGY  Female  Gene Products,
       gag/ANALYSIS/GENETICS  Immunodeficiency Virus,
       Feline/GENETICS/IMMUNOLOGY/*ISOLATION &  PURIF  Immunoenzyme
       Techniques/VETERINARY  Kidney/PATHOLOGY/*VIROLOGY  Kidney
       Diseases/*VETERINARY/VIROLOGY  Male  Polymerase Chain
       Reaction/VETERINARY  RNA, Viral/ANALYSIS  Support, Non-U.S. Gov't  Virus
       Cultivation/VETERINARY  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).