Document 0796
 DOCN  M95A0796
 TI    Requirements for activation of CD8+ murine T cells. I. Development of
       cytolytic activity.
 DT    9510
 AU    Cronin DC 2nd; Lancki DW; Fitch FW; Department of Pathology, University
       of Chicago, IL 60637, USA.
 SO    Immunol Res. 1994;13(4):215-33. Unique Identifier : AIDSLINE
       MED/95341106
 AB    Cytolytic effector function fails to develop if proliferation of
       allospecific cytolytic T lymphocyte precursors is inhibited, but the
       requirements for generation of cytolytic activity have not been fully
       defined. In contrast, the cytolytic effector function of cytolytic T
       lymphocyte clones does not change during the cell cycle, and the level
       of cytolytic activity is independent of cellular proliferation. The
       requirement for proliferation by primary responding populations may
       reflect the need for clonal expansion of a few inherently cytolytic
       effector cells in order to reach a threshold number which can readily be
       detected in conventional cytolytic assays. Alternatively, proliferation
       may be required for cytolytic T lymphocyte precursors to differentiate
       into mature, functional cytolytic cells. Using CD8+ T cells which
       express an antigen-specific transgenic alpha/beta T cell receptor, we
       have studied the requirements for acquisition of cytolytic capacity.
       Stimulation of the T cell receptor alone appears to be sufficient to
       render naive, CD8+ transgenic T cells sensitive to the growth effects of
       interleukin-2 (IL-2), and in some circumstances to interleukin-4 (IL-4),
       but not to induce either lymphokine production or cytolytic activity.
       Costimulatory molecules expressed by allogenic stimulating cells appear
       to be required for lymphokine production, and CD8+ transgenic T cells
       initially appear to secrete only IL-2 and interferon-gamma. Stimulation
       of the T cell receptor of naive, CD8+ transgenic T cells appears to
       induce cytolytic activity only if cell proliferation occurs, either in
       response to IL-2 produced by the stimulated cells themselves when
       costimulatory molecules are present, or to IL-2 or IL-4 from exogenous
       sources if costimulatory molecules are absent.
 DE    Animal  Antibodies, Monoclonal/PHARMACOLOGY  Antigens, CD8/DRUG
       EFFECTS/IMMUNOLOGY  Aphidicolin/PHARMACOLOGY  Cell Cycle  Cells,
       Cultured  Cytotoxicity, Immunologic  CD8-Positive T-Lymphocytes/DRUG
       EFFECTS/*IMMUNOLOGY  Female  Hydroxyurea/PHARMACOLOGY  Interferon Type
       II/SECRETION  Interleukin-2/PHARMACOLOGY/SECRETION
       Interleukin-4/PHARMACOLOGY  *Lymphocyte Transformation/DRUG EFFECTS
       Mice  Mice, Inbred C57BL  Mice, Inbred DBA  Mice, Transgenic
       Mimosine/PHARMACOLOGY  Receptors, Antigen, T-Cell/DRUG
       EFFECTS/IMMUNOLOGY  Receptors, Antigen, T-Cell, alpha-beta/DRUG
       EFFECTS/IMMUNOLOGY  Recombinant Proteins/PHARMACOLOGY  Serine
       Proteinases/ANALYSIS  Spleen/ENZYMOLOGY/IMMUNOLOGY  Support, U.S. Gov't,
       P.H.S.  T-Lymphocytes, Cytotoxic/DRUG EFFECTS/IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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