Document 0817
 DOCN  M95A0817
 TI    Genomic fingerprinting of Bartonella species by repetitive element PCR
       for distinguishing species and isolates.
 DT    9510
 AU    Rodriguez-Barradas MC; Hamill RJ; Houston ED; Georghiou PR; Clarridge
       JE; Regnery RL; Koehler JE; Section of Infectious Diseases, Medical
       Service, Veterans Affairs; Medical Center, Houston, TX 77030, USA.
 SO    J Clin Microbiol. 1995 May;33(5):1089-93. Unique Identifier : AIDSLINE
       MED/95340726
 AB    Repetitive-element PCR (rep-PCR) with primers based on repetitive
       extragenic palindromic (REP) and enterobacterial repetitive intergenic
       consensus (ERIC) repeated DNA sequences was used for genomic
       finger-printing of Bartonella species. This technique was applied by
       using either extracted genomic DNA or preparations of whole bacterial
       cells directly. PCR fingerprints with either the REP-based primers
       (REP-PCR) or primers based on the ERIC repeat (ERIC-PCR) revealed
       species-specific band patterns for the various Bartonella isolates. DNA
       fingerprints obtained from rep-PCR of extracted genomic DNA or from
       preparations of whole cells yielded comparable patterns. ERIC-PCR
       banding patterns were less complex than those obtained by REP-PCR but
       allowed better discrimination between strains within species. By
       combining results of REP-PCR and ERIC-PCR, five different fingerprint
       profiles were identified among 17 isolates of Bartonella henselae, but
       only one profile was identified among the five isolates of Bartonella
       quintana. Other Bartonella species yielded distinct rep-PCR
       fingerprints. rep-PCR is a useful technique for identification of
       Bartonella organisms to the species level and offers the advantage of
       ease of performance, with only small quantities of cells needed for the
       whole-cell procedure. This technique also appears to be useful for
       subtyping B. henselae isolates.
 DE    Animal  AIDS-Related Opportunistic Infections/MICROBIOLOGY  Bacterial
       Typing Techniques  Bartonella/CLASSIFICATION/*GENETICS/ISOLATION & PURIF
       Bartonella Infections/MICROBIOLOGY  Cats  Consensus Sequence  DNA
       Fingerprinting/*METHODS  DNA Primers  DNA, Bacterial/GENETICS
       Evaluation Studies  Human  Polymerase Chain Reaction/*METHODS
       Repetitive Sequences, Nucleic Acid  Species Specificity  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).