Document 0818
 DOCN  M95A0818
 TI    DNA fingerprinting and phenotyping of Mycobacterium tuberculosis
       isolates from human immunodeficiency virus (HIV)-seropositive and
       HIV-seronegative patients in Tanzania.
 DT    9510
 AU    Yang ZH; Mtoni I; Chonde M; Mwasekaga M; Fuursted K; Askgard DS;
       Bennedsen J; de Haas PE; van Soolingen D; van Embden JD; et al;
       Mycobacteria Department, Statens Seruminstitut, Copenhagen,; Denmark.
 SO    J Clin Microbiol. 1995 May;33(5):1064-9. Unique Identifier : AIDSLINE
       MED/95340721
 AB    With the purpose of determining whether the risk of infection with a
       particular clone of Mycobacterium tuberculosis is influenced by the
       human immunodeficiency virus (HIV) status of the host, we analyzed and
       compared 68 mycobacterial isolates obtained from HIV-seropositive
       patients with tuberculosis (TB) in Dar es Salaam, Tanzania, with 66
       mycobacterial isolates obtained from HIV-seronegative patients with TB
       in the same geographical region by using both DNA fingerprinting and
       classical phenotyping methods. One hundred one different IS6110
       fingerprinting patterns were observed in the 134 isolates. The level of
       diversity of the DNA fingerprints observed in the HIV-seropositive group
       was comparable to the level of the diversity observed in the
       HIV-seronegative group. Resistance to a single anti-TB drug was found in
       8.8% of the tested isolates, and 3.2% of the isolates were resistant to
       more than one anti-TB drug. The drug susceptibility profiles were not
       significantly difference between the two groups of isolates compared in
       the present study. Phenotypic characteristics which classify M.
       tuberculosis strains as belonging to the Asian subgroup correlated with
       a low IS6110 copy number per isolate. However, the occurrence of Asian
       subgroup strains was not associated with the HIV status of the patients.
       The results of the study suggested an equal risk of infection with a
       defined M. tuberculosis clone for HIV-seropositive and HIV-seronegative
       individuals.
 DE    Adolescence  Adult  Aged  AIDS-Related Opportunistic
       Infections/*COMPLICATIONS/EPIDEMIOLOGY/  MICROBIOLOGY  Comparative Study
       DNA Fingerprinting  DNA Insertion Elements  Epidemiology, Molecular
       Human  HIV Infections/*COMPLICATIONS/EPIDEMIOLOGY/MICROBIOLOGY  HIV
       Seronegativity  HIV
       Seropositivity/COMPLICATIONS/EPIDEMIOLOGY/MICROBIOLOGY  Middle Age
       Mycobacterium tuberculosis/CLASSIFICATION/*GENETICS/ISOLATION &  PURIF
       Phenotype  Polymorphism, Restriction Fragment Length  Support, Non-U.S.
       Gov't  Tanzania/EPIDEMIOLOGY
       Tuberculosis/*COMPLICATIONS/EPIDEMIOLOGY/MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).