Document 0825
 DOCN  M95A0825
 TI    Induction of peptide-specific primary cytotoxic T lymphocyte responses
       from human peripheral blood.
 DT    9510
 AU    Plebanski M; Allsopp CE; Aidoo M; Reyburn H; Hill AV; Nuffield
       Department of Medicine, University of Oxford, John; Radcliffe Hospital,
       GB.
 SO    Eur J Immunol. 1995 Jun;25(6):1783-7. Unique Identifier : AIDSLINE
       MED/95339899
 AB    Various protocols were developed and compared for eliciting specific
       cytotoxic T lymphocyte (CTL) cell lines from the unselected human
       peripheral blood mononuclear cells of naive donors. Interleukin-7 and
       CD4+ T cells primed in vitro by keyhole limpet hemocyanin were shown to
       act together in the generation of these responses. Primary responses
       were consistently induced with a variety of different HLA class
       I-binding malarial peptides. Primary CTL responses could be induced from
       unselected CD8+ and from CD45RA+CD8+ T cells. The CTL lines derived from
       these naive donors were CD8+ and demonstrated a high level of HLA class
       I-restricted killing for > 3 months after priming in vitro. They were
       also able to recognize and kill targets infected with a recombinant
       vaccinia virus containing the full-length antigen. In addition, this
       same protocol enhanced up to fourfold the levels of secondary CTL
       responses induced. The optimal method presented for naive cytotoxic T
       cell stimulation is simple, rapid and generally applicable and should
       provide a useful tool for both basic research and human therapy.
 DE    Cell Line  *Cytotoxicity, Immunologic  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Histocompatibility Antigens Class
       I/METABOLISM  Human  Leukocytes, Mononuclear/IMMUNOLOGY  Lymphocyte
       Transformation  Peptides/IMMUNOLOGY  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

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