Document 0828
 DOCN  M95A0828
 TI    Cloning of ATAC, an activation-induced, chemokine-related molecule
       exclusively expressed in CD8+ T lymphocytes.
 DT    9510
 AU    Muller S; Dorner B; Korthauer U; Mages HW; D'Apuzzo M; Senger G; Kroczek
       RA; Molecular Immunology, Robert-Koch-Institute, Berlin, Germany.
 SO    Eur J Immunol. 1995 Jun;25(6):1744-8. Unique Identifier : AIDSLINE
       GENBANK/X86474
 AB    A cDNA clone, designated ATAC, was isolated from a collection of human T
       cell activation genes. Analysis of tissue distribution determined that
       ATAC mRNA of approximately 0.9 kb is exclusively expressed in activated
       CD8+ T cells. Induction of the ATAC gene requires stimulation by both
       phorbol 12-myristate 13-acetate and Ca2+ ionophore A23187 (two-signal
       gene) and is fully abrogated by the immunosuppressive agent cyclosporin
       A. Upon stimulation, ATAC mRNA is detectable within 30 min, maximal
       expression is seen after 4 h. The polypeptide encoded by the open
       reading frame of ATAC mRNA is 114 amino acids long with a calculated
       M(r) of 12.52 kDa. The structural features predict the cleavage and
       secretion of a mature ATAC protein of approximately 10 kDa from the
       12.52-kDa precursor. ATAC is highly similar to a very recently
       identified murine molecule designated lymphotactin both at the cDNA
       (73.8% identity) and the protein (61.4% identity) levels, and related to
       members of the C-C and C-X-C chemokine families. Two variants of the
       ATAC protein were expressed and tested for chemotaxis and Ca2+ release
       on a variety of target cells. The ATAC gene was located to chromosome
       1q23.
 DE    Amino Acid Sequence  Base Sequence  Chromosome Banding  Chromosome
       Mapping  Chromosomes, Human, Pair 1/GENETICS  Cloning, Molecular
       Cytokines/BIOSYNTHESIS/*GENETICS  CD8-Positive T-Lymphocytes/*METABOLISM
       DNA, Complementary/ISOLATION & PURIF  Human  Lymphocyte
       Transformation/GENETICS  Molecular Sequence Data  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).