Document 0830
 DOCN  M95A0830
 TI    Selective variations in vivo of VH3 and VH1 gene family expression in
       peripheral B cell IgM, IgD and IgG during HIV infection.
 DT    9510
 AU    David D; Demaison C; Bani L; Zouali M; Theze J; Departement
       d'Immunologie, Institut Pasteur, Paris, France.
 SO    Eur J Immunol. 1995 Jun;25(6):1524-8. Unique Identifier : AIDSLINE
       MED/95339862
 AB    We have analyzed the expression of VH gene families in IgM, IgD and IgG
       of peripheral blood B cells from a group of HIV-infected patients.
       CD19+CD20+ cells were purified and anchored reverse
       transcriptase-polymerase chain reaction products were hybridized with VH
       gene family probes. IgM, IgD and IgG that expressed a VH3 gene family
       segment, were decreased in patients with low CD4 counts and to a greater
       extend in patients with AIDS symptoms (up to 85% for IgG) compared to
       adult healthy donors. This was correlated with elevated levels of IgM
       and IgG encoded by a VH1 gene family segment (around 60% for IgG). These
       results confirm and extend previous work that has detected the VH3 gene
       family under-representation in HIV infection. Here, we show that, in
       vivo, this phenomenon actually affects the different B cell populations
       of the peripheral blood: IgM+ or IgG+ B cells and also IgM+IgD+ naive B
       cells. In the course of HIV infection, this results in their gradual
       depletion. Data presented here strengthen the hypothesis that a B-cell
       superantigen exists in HIV infection. These pronounced variations of the
       normally most-expressed VH gene family may be related to B cell
       abnormalities detected in HIV-infected patients.
 DE    Adult  B-Lymphocytes/*IMMUNOLOGY/VIROLOGY  Base Sequence  Gene
       Expression Regulation  Human  HIV Infections/GENETICS/*IMMUNOLOGY
       IgD/BIOSYNTHESIS/*GENETICS  IgG/BIOSYNTHESIS/*GENETICS
       IgM/BIOSYNTHESIS/*GENETICS  Immunoglobulin Variable Region/GENETICS
       Molecular Sequence Data  RNA, Messenger/ANALYSIS  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

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