Document 0842
 DOCN  M95A0842
 TI    In vitro activation leads to the binding of T-cell markers to the
       surface of B-lymphocytes.
 DT    9510
 AU    Rabinowitz R; Massiah E; Hadar R; Schlesinger M; Hubert H. Humphrey
       Center for Experimental Medicine, Hebrew; University, Hadassah Medical
       School, Jerusalem, Israel.
 SO    Clin Immunol Immunopathol. 1995 Aug;76(2):148-54. Unique Identifier :
       AIDSLINE MED/95339607
 AB    The aim of the present study was to determine whether activation of
       human T-cells in vitro results in the expression of markers
       characteristic for T-cells on the surface of B-lymphocytes and to
       correlate antigenic changes with the release of soluble T-cell antigens.
       Peripheral blood lymphocytes (PBL) were exposed in vitro to
       phytohemagglutinin (PHA) for 3 days. Changes in the phenotype of the
       cells were determined by flow cytometry and the level of soluble T-cell
       antigens was assessed by ELISA. PHA-activated PBL released elevated
       quantities of soluble CD2, CD4, and CD8 compared with control cultures.
       Following PHA stimulation the proportion of CD4+ CD8+ and HLA-DR+ cells
       increased. In addition, after 3 days of activation with PHA about 80% of
       the CD19+ cells (B-cells) reacted with F(ab)2 fragments of CD2
       monoclonal antibodies (MoAbs). A high proportion of B-cells in activated
       cultures also reacted with F(ab)2 fragments of anti-CD8 MoAb and
       anti-CD4 MoAb. The removal of either CD4+ or CD8+ T-cells from the
       cultures prior to stimulations with PHA drastically reduced the
       proportion of B-cells expressing CD4 or CD8, respectively. The
       attachment of T-cell markers to the surface B-lymphocytes may constitute
       a new mechanism for B-cell regulation by T-cells.
 DE    Antigens, CD/*BIOSYNTHESIS  Antigens, CD2/BIOSYNTHESIS  Antigens,
       CD4/BIOSYNTHESIS  Antigens, CD8/BIOSYNTHESIS  Antigens,
       Surface/*PHYSIOLOGY  B-Lymphocytes/*METABOLISM  Biological
       Markers/ANALYSIS  Cells, Cultured  CD4-Positive T-Lymphocytes/IMMUNOLOGY
       CD8-Positive T-Lymphocytes/IMMUNOLOGY  Human  Lymphocyte
       Transformation/IMMUNOLOGY  Phytohemagglutinins/PHARMACOLOGY  Protein
       Binding/PHYSIOLOGY  Solubility  Support, Non-U.S. Gov't
       T-Lymphocytes/DRUG EFFECTS/*IMMUNOLOGY/*METABOLISM  JOURNAL ARTICLE

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