Document 0843
 DOCN  M95A0843
 TI    Determination of CD4+ and CD8+ lymphocytes with the cytosphere assay: a
       comparative study with flow cytometry and the immunoalkaline phosphatase
       method.
 DT    9510
 AU    Gernow A; Lisse IM; Bottiger B; Christensen L; Brattegaard K; Department
       of Pathology, Hvidovre University Hospital,; Copenhagen, Denmark.
 SO    Clin Immunol Immunopathol. 1995 Aug;76(2):135-41. Unique Identifier :
       AIDSLINE MED/95339605
 AB    The proportion and absolute numbers of CD4+ and CD8+ lymphocytes in
       peripheral blood were determined using a new manual method, the
       cytosphere assay (CA). This method uses small latex beads coated with
       monoclonal antibodies directed against the CD4 and CD8 receptors,
       respectively. The CA was compared with two other methods for
       determination of T lymphocyte subsets, flow cytometry (FC) and the
       immunoalkaline phosphatase (IA) method, by testing HIV-seropositive and
       HIV-seronegative samples from Denmark (44) and Ivory Coast (79). For
       HIV-seropositive samples, both the proportion and the absolute number of
       CD4+ lymphocytes determined by CA showed a good correlation with results
       obtained by FC (correlation coefficients were 0.92 and 0.74 in Denmark
       and The Ivory Coast, respectively) and IA (correlation coefficients were
       0.94 and 0.66 in Denmark and The Ivory Coast, respectively). However,
       for HIV-seronegative samples the corresponding correlation coefficients
       were low. CD4% determinations deviated more from FC counts at higher CD4
       counts than at lower levels for both seronegative and seropositive
       individuals. In conclusion, the CA performed best for samples from
       HIV-infected individuals. Before a more general utilization of the
       method, it is necessary to improve its repeatability and standardize its
       performance at all levels of CD4+ T cells.
 DE    Alkaline Phosphatase/*DIAGNOSTIC USE  Comparative Study  Costs and Cost
       Analysis  CD4-Positive T-Lymphocytes/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Female  Flow Cytometry/ECONOMICS/*METHODS
       Human  HIV Infections/IMMUNOLOGY  Immunologic
       Techniques/ECONOMICS/STANDARDS  Male  *Microspheres  Sensitivity and
       Specificity  Statistics/METHODS  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

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