Document 0875
 DOCN  M95A0875
 TI    The role of spleen in the pathogeny of aplastic anemia related to
       increased number of CD3+ CD8+ FcR+ cells.
 DT    9510
 AU    Roman S; Grigoriu G; Puscariu T; Fagarasanu D; Berceanu S; Center of
       Immunology, St. Nicolau Institute of Virology,; Bucharest, Romania.
 SO    Rom J Intern Med. 1994 Oct-Dec;32(4):275-82. Unique Identifier :
       AIDSLINE MED/95338161
 AB    The role of spleen in the pathogeny of aplastic anemia (A.A.) related to
       excessive suppression, and the value of splenectomy in the treatment of
       this disorder is still debated and unclear. In an attempt to find out
       why some patients respond to surgery and others do not, an immunologic
       study was carried out in 16 patients with aplastic anemia. Lymphocytes
       surface markers CD3, CD4, CD8, HLA-DR, Fc receptors (FcR) and CD4/CD8
       ratio were determined before and after splenectomy in the patients'
       peripheral blood, and in the spleen. In addition, the number of
       granulo-monocytic colony forming cells (GM-CFC) before and after
       splenectomy was estimated. Nine of the cases showed increased CD3+ CD8+
       FcR+ cells, reversed CD4/CD8 ratios (both, in peripheral blood and in
       spleen), and a low number of GM-CFC. In all these cases, splenectomy
       induced an improvement of the clinical, hematological, and immunological
       parameters, thus suggesting that spleen represents an important
       reservoir for CD3+ CD8+ FcR+ cells, which seem to exert a suppressor
       effect on the hematopoietic progenitors. In splenectomized patients who
       did not respond to surgery, the pathogenic mechanism was probably
       related to defective help (3 cases with low values of CD4+ cells), to
       defective suppression (2 cases with decreased number of CD8+ cells), to
       a stem cell defect or a deficiency in the stem cell microenvironment (2
       cases with normal helper/suppressor ratio). These observations support
       the conclusion that splenectomy is indicated and may be successful only
       when the phenotypic markers show an increased number of CD3+ CD8+ FcR+
       cells.
 DE    Anemia, Aplastic/*ETIOLOGY/*IMMUNOLOGY/SURGERY  Antigens, CD3/*BLOOD
       Biological Markers/BLOOD  Colony-Forming Units Assay  Comparative Study
       CD4-CD8 Ratio  CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Human  HLA-DR Antigens/BLOOD  Leukocytes,
       Mononuclear/IMMUNOLOGY  Receptors, Fc/ANALYSIS/*IMMUNOLOGY
       Spleen/IMMUNOLOGY/*PHYSIOPATHOLOGY  Splenectomy  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).