Document 0882
 DOCN  M95A0882
 TI    Cytokine production by highly purified human CD8+ T cells.
 DT    9510
 AU    Li Y; Richards D; Noble A; Kemeny DM; Department of Allergy and
       Respiratory Medicine, Guy's and St.; Thomas's Hospital, London, UK.
 SO    Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):354-5. Unique Identifier
       : AIDSLINE MED/95337785
 AB    We have systematically investigated the capacity of highly purified
       human peripheral CD8+ T cells to produce interleukin (IL)-4 and
       interferon (IFN)-gamma when triggered by different stimuli. CD8+ T cells
       were isolated from peripheral blood by positive selection to > 99%
       purity and stimulated with one of three different stimuli:
       phytohaemagglutinin (PHA) and IL-2, phorbol myristate acetate (PMA) and
       ionomycin, and plate-bound anti CD3 and PMA. On their own, ionomycin and
       IL-2 failed to stimulate significant CD8+ T cell proliferation while
       PHA, plate-bound anti-CD3 and PMA induced weak proliferation. A
       combination of PHA and IL-2, PMA and ionomycin, or plate-bound anti-CD3
       and PMA all induced vigorous CD8+ T cell proliferation. IFN-gamma was
       produced following all three stimuli, but was greatest from cells
       cultured with PMA and ionomycin. However, IL-4 secretion was only
       detected in cell cultures stimulated with PMA and ionomycin. These
       results indicate that, with sufficient stimulation, human CD8+ T cells
       have the potential to produce Th2 as well as Th1 cytokines.
 DE    Antigens, CD3/PHARMACOLOGY  Cell Separation  Comparative Study
       CD8-Positive T-Lymphocytes/DRUG EFFECTS/*METABOLISM  Human  Interferon
       Type II/*BIOSYNTHESIS  Interleukin-2/PHARMACOLOGY
       Interleukin-4/*BIOSYNTHESIS  Ionomycin/PHARMACOLOGY  Lymphocyte
       Transformation/DRUG EFFECTS  Support, Non-U.S. Gov't
       Tetradecanoylphorbol Acetate/PHARMACOLOGY  JOURNAL ARTICLE

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