Document 0886
 DOCN  M95A0886
 TI    A conserved motif in the promoters of several cytokines expressed by
       human Th2-type lymphocytes.
 DT    9510
 AU    Staynov DZ; Cousins DJ; Lee TH; Department of Allergy and Respiratory
       Medicine, UMDS, Guy's; Hospital, University of London, UK.
 SO    Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):217-9. Unique Identifier
       : AIDSLINE MED/95337734
 AB    We have recently found a novel conserved motif in the promoters of
       several T-cell-expressed cytokines [human interleukin-2, -4, 5 and -13
       and human and mouse granulocyte/macrophage-colony stimulating factor
       (GM-CSF)]. It contains a core sequence CTTGG ... CCAAG which is present
       as part of larger palindromic sequences in each gene. This suggest that
       they may interact with a new family of trans-acting factors. In
       transfection assays, the human GM-CSF element has a strong positive
       effect on the expression of a reporter gene by the human T cell line
       Jurkat J6 upon stimulation. In DNA mobility shift assays, this sequence
       can give either six different specific bands which are competed out by
       different parts of the sequence or one specific band which is competed
       out by each of the inverted repeats, depending on the reconstitution
       conditions. In different genes, the core sequences are separated by
       integer numbers of helical turns. Considering the strong positive
       regulatory effect of this element and its presence in several
       T-cell-expressed cytokine genes, it may be crucial to the coordinated
       expression of these cytokines in T helper cells.
 DE    Animal  Base Sequence  Comparative Study  *Consensus Sequence
       Cytokines/*GENETICS  Evolution  Gene Expression Regulation, Leukemic
       Genes, Reporter  Granulocyte-Macrophage Colony-Stimulating
       Factor/GENETICS  Human  Interleukins/GENETICS  Leukemia, T-Cell,
       Acute/PATHOLOGY  Mice  Molecular Sequence Data  *Promoter Regions
       (Genetics)  Recombinant Fusion Proteins/BIOSYNTHESIS  Repetitive
       Sequences, Nucleic Acid  *Sequence Homology, Nucleic Acid  Support,
       Non-U.S. Gov't  Th2 Cells/*METABOLISM  Transcription Factors/METABOLISM
       Transfection  Tumor Cells, Cultured  JOURNAL ARTICLE  REVIEW  REVIEW,
       TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).