Document 0887
 DOCN  M95A0887
 TI    Interleukin-4 and interferon-gamma regulate differentiation of CD8+ T
       cells into populations with divergent cytokine profiles.
 DT    9510
 AU    Noble A; Kemeny DM; Department of Allergy and Respiratory Medicine,
       Guy's and St; Thomas's Hospital, London, UK.
 SO    Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):186-8. Unique Identifier
       : AIDSLINE MED/95337723
 AB    The ability of CD8+ T cells to synthesise and secrete cytokines before
       and after culture with interleukin (IL)-4 and anti-interferon
       (IFN)-gamma was investigated. Purified rat splenic CD8+ and CD4+ T cells
       and CD4-CD8- cells were stimulated with phorbol myristate acetate (PMA)
       and ionomycin for 6 h and expression of mRNA for IL-2, IL-4, IL-5, IL-6,
       IL-10 and IFN-gamma determined using a quantitative PCR technique.
       Secreted IFN-gamma and IL-2 were measured by ELISA and bioassay,
       respectively, 24 h after restimulation. The highest levels of IL-4, IL-5
       and IFN-gamma mRNA were expressed by CD8+ T cells, the highest levels of
       message for IL-2 were expressed by CD4+ T cells and the highest levels
       of message for IL-6 and IL-10 by CD4-CD8- splenocytes. Purified rat
       splenic CD8+ T cells were cultured for 6 days with PMA, ionomycin and
       IL-2 with or without IL-4 or anti-IFN-gamma and restimulated with PMA
       and ionomycin. Those CD8+ T cells cultured with IL-4 expressed increased
       levels of IL-4, IL-5 and reduced levels of IL-2 and IL-6 mRNA, while
       CD8+ T cells cultured with IL-4 and anti-IFN-gamma expressed increased
       levels of mRNA for IL-10 and IFN-gamma. Comparable effects were seen for
       secreted IFN-gamma and IL-2. These results indicate that CD8+ T cells
       have the potential to produce large amounts of Th2 cytokines and are
       able to differentiate into different subpopulations with distinct
       cytokine profiles under the control of IL-4 and IFN-gamma.
 DE    Animal  Cell Differentiation/DRUG EFFECTS  Cells, Cultured  Comparative
       Study  CD8-Positive T-Lymphocytes/*DRUG EFFECTS/IMMUNOLOGY/SECRETION
       Interferon Type II/BIOSYNTHESIS/*PHARMACOLOGY/SECRETION
       Interleukin-4/*PHARMACOLOGY  Interleukins/BIOSYNTHESIS/SECRETION
       Ionomycin/PHARMACOLOGY  Lymphocyte Transformation/DRUG EFFECTS
       Lymphokines/BIOSYNTHESIS/*SECRETION  Rats  RNA, Messenger/GENETICS
       Spleen/CYTOLOGY  Support, Non-U.S. Gov't  Tetradecanoylphorbol
       Acetate/PHARMACOLOGY  JOURNAL ARTICLE

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