Document 0888
 DOCN  M95A0888
 TI    Characterization of allergen (Bet v 1)-specific T cell lines and clones
       from non-allergic individuals.
 DT    9510
 AU    Ebner C; Siemann U; Najafian N; Scheiner O; Kraft D; Institute of
       General and Experimental Pathology, University of; Vienna, Austria.
 SO    Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):183-5. Unique Identifier
       : AIDSLINE MED/95337722
 AB    The immune response towards allergens in non-allergic healthy
       individuals was investigated. T cell lines (TCL) with specificity for
       Bet v 1, the major birch pollen allergen, were established and analysed
       for epitope specificity. 49 T cell clones (TCC) specific for Bet v 1
       were isolated from TCLs. All TCCs revealed the Th phenotype. Cytokine
       production in response to specific stimulation revealed a majority of Th
       clones producing interleukin (IL)-4 and interferon (IFN)-gamma; however,
       most TCCs revealed a low IL-4/IFN-gamma ratio. Immunoblot revealed Bet v
       1-specific IgG in non-allergic individuals whereas no IgE could be
       detected. Our results indicate that T cells from allergic and
       non-allergic individuals recognize the same epitopes on allergenic
       molecules, leading to activation, which then results in a differential
       production of cytokines and consequently to differential isotype
       switching in allergen-specific B cells.
 DE    Allergens/*IMMUNOLOGY  Antigenic Determinants/IMMUNOLOGY  Cell Line
       Clone Cells/IMMUNOLOGY  Female  Human  IgG/IMMUNOLOGY  Immunity,
       Cellular  Immunoglobulin Class Switching  Interferon Type
       II/BIOSYNTHESIS  Interleukin-4/BIOSYNTHESIS  Male  Peptide
       Fragments/IMMUNOLOGY  Plant Proteins/*IMMUNOLOGY  Pollen/*IMMUNOLOGY
       Support, Non-U.S. Gov't  T-Lymphocyte Subsets/*IMMUNOLOGY  Th1
       Cells/IMMUNOLOGY  Th2 Cells/IMMUNOLOGY  Trees  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).