Document 0919
 DOCN  M95A0919
 TI    Flow cytometric three-color determination of CD4 T-lymphocytes on blood
       specimens from AIDS patients who have a large number of contaminating
       non-lymphocytes.
 DT    9510
 AU    Pattanapanyasat K; Pengruangrojanachai V; Thepthai C; Suwanagool S; Wasi
       C; Thalassemia Center, Faculty of Medicine Siriraj Hospital, Mahidol;
       University, Bangkok, Thailand.
 SO    Asian Pac J Allergy Immunol. 1994 Dec;12(2):105-9. Unique Identifier :
       AIDSLINE MED/95336545
 AB    A three-color flow cytometric determination of CD4 T-lymphocytes on
       whole blood specimens from AIDS patients which contain a high proportion
       of non-lymphocyte elements is described. Peripheral blood cells were
       stained by a three-color method using monoclonal antibodies conjugated
       respectively with fluorescein isothiocyanate (FITC)-CD3, phycoerythrin
       (PE)-CD4 and peridinin chlorophyll protein (PerCP)-CD45. CD45 stains all
       leukocytes with the highest fluorescence expression of CD45 antigen in
       lymphocytes. By combining light scatter with CD45 in the fluorescence 3
       (FL3) channel, a light scattering window can be drawn to include almost
       all bright CD45 lymphocytes. This live gate of lymphocytes was then
       acquired and analysed simultaneously using other irrelevant two-color
       (FITC/PE) antibodies of CD3 and CD4 in the FITC and PE channels,
       respectively. This method is easy and straightforward, and gives
       successful analysis of CD4 T-lymphocytes in AIDS blood specimens
       contaminated with an unusually large number of non-lymphocytic cells.
 DE    Acquired Immunodeficiency Syndrome/*BLOOD/IMMUNOLOGY  Antibodies,
       Monoclonal  Antigens, CD3/ANALYSIS  Antigens, CD4/ANALYSIS  Antigens,
       CD45/ANALYSIS  CD4 Lymphocyte Count/METHODS  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY  Flow Cytometry/*METHODS  Fluorescent Dyes
       Human  HIV Seropositivity/*BLOOD/IMMUNOLOGY  Immunophenotyping  Male
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).