Document 0090
 DOCN  M95B0090
 TI    Protective immunity to Porphyromonas gingivalis infection in a murine
       model.
 DT    9511
 AU    Bird PS; Gemmell E; Polak B; Paton RG; Sosroseno W; Seymour GJ;
       Department of Dentistry, University of Queensland, Australia.
 SO    J Periodontol. 1995 May;66(5):351-62. Unique Identifier : AIDSLINE
       MED/95348944
 AB    The mouse abscess model has been used extensively to demonstrate
       protection after challenge with periodontopathic organisms. In the
       present study, an outer membrane (OM) preparation of P. gingivalis ATCC
       33277 was used to immunize BALB/c mice prior to challenge with live P.
       gingivalis organisms. This OM preparation, particularly at the highest
       dose level of 100 micrograms/immunization, was able to induce high
       levels of specific antibody and subsequent protective immunity.
       Protection in all immunized mice was noted by the rapid healing of the
       primary lesions, a low incidence of secondary lesions, and, in the
       highest dose group, an absence of septicemia. Non-immunized animals
       demonstrated a slower development as well as healing of primary lesions,
       with higher numbers and larger sizes of secondary lesions. Weight loss
       and behavior patterns such as hunched bodies, ruffled hair, and
       stiffness of the hind legs were particularly noted in this group.
       Depletion of CD4 T cells in mice prior to immunization with 100
       micrograms P. gingivalis OM resulted in significantly depressed serum
       levels of anti-P. gingivalis antibody and an increase in the physical
       signs of disease compared with both the immunized and control groups.
       Western blot analysis demonstrated three antigen bands (63.3, 50.1, and
       45.1) recognized by all immunized groups and also the control
       non-immunized group, although the latter recognition occurred only after
       challenge. A further antigen band of 36.1 kDa was recognized by sera
       from the highest dose group only. This study has demonstrated the
       ability of P. gingivalis OM to provide protection against challenge with
       live P. gingivalis organisms. The increased physical signs of disease
       seen in the CD4 depleted animals compared with the control group not
       only illustrate the protective role of serum antibody, but also suggest
       a possible role for T cell mechanisms in control of the lesion locally.
       The ability of specific OM antigens to provide similar protective
       immunity remains to be ascertained.
 DE    Analysis of Variance  Animal  Antibodies, Bacterial/BIOSYNTHESIS/BLOOD
       Antigens, Bacterial/ISOLATION & PURIF  Bacterial Outer Membrane
       Proteins/IMMUNOLOGY  *Bacterial Vaccines  Bacteroidaceae
       Infections/IMMUNOLOGY/*PREVENTION & CONTROL  Blotting, Western  CD4
       Lymphocyte Count  Electrophoresis, Polyacrylamide Gel  Enzyme-Linked
       Immunosorbent Assay  Female  Male  Mice  Mice, Inbred BALB C
       Periodontal Abscess/IMMUNOLOGY/MICROBIOLOGY/*PREVENTION & CONTROL
       Porphyromonas gingivalis/*IMMUNOLOGY  Support, Non-U.S. Gov't
       Vaccination  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).