Document 0107
 DOCN  M95B0107
 TI    Decreased activation of the respiratory burst in neutrophils from AIDS
       patients with previous Pneumocystis carinii pneumonia.
 DT    9511
 AU    Laursen AL; Rungby J; Andersen PL; Department of Infectious Diseases,
       Marselisborg Hospital, Aarhus,; Denmark.
 SO    J Infect Dis. 1995 Aug;172(2):497-505. Unique Identifier : AIDSLINE
       MED/95348550
 AB    Neutrophils from human immunodeficiency virus (HIV)-negative blood
       donors, asymptomatic HIV-positive patients, AIDS patients with previous
       Pneumocystis carinii pneumonia (PCP), and AIDS patients without previous
       PCP were compared for their ability to activate the respiratory burst,
       measured as luminol-amplified chemiluminescence. P. carinii,
       Staphylococcus aureus, phorbol-12-myristate-13-acetate, and FMLP were
       used to stimulate the neutrophils. When stimulated with P. carinii,
       neutrophils from PCP patients had a significantly lower response than
       the other groups, whereas no difference was found when S aureus was
       used. A somewhat but not significantly lower response to P. carinii was
       also seen in non-PCP patients compared with HIV-negative donors. Priming
       of the neutrophils with recombinant granulocyte colony-stimulating
       factor (G-CSF) or recombinant human granulocyte-macrophage (GM)-CSF
       corrected this defect. A similar effect of these cytokines was seen on
       phagocytosis, whereas the chemiluminescence in unprimed cells did not
       correlate with phagocytosis.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS/*IMMUNOLOGY  Adult
       Animal  Case-Control Studies  Chemiluminescence  Female  Granulocyte
       Colony-Stimulating Factor/PHARMACOLOGY  Granulocyte-Macrophage
       Colony-Stimulating Factor/PHARMACOLOGY  Human  HIV Seronegativity/DRUG
       EFFECTS/PHYSIOLOGY  HIV Seropositivity/COMPLICATIONS/METABOLISM
       Kinetics  Leukocyte Count/DRUG EFFECTS  Male  Middle Age
       N-Formylmethionine Leucyl-Phenylalanine/PHARMACOLOGY  Neutrophils/DRUG
       EFFECTS/*IMMUNOLOGY/ULTRASTRUCTURE  Phagocytosis/DRUG EFFECTS
       Pneumocystis carinii/METABOLISM/ULTRASTRUCTURE  Pneumonia, Pneumocystis
       carinii/COMPLICATIONS/*IMMUNOLOGY  Rats  Recombinant
       Proteins/PHARMACOLOGY  Respiratory Burst/DRUG EFFECTS/*PHYSIOLOGY
       Staphylococcus aureus/METABOLISM  Support, Non-U.S. Gov't
       Tetradecanoylphorbol Acetate/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).