Document 0108
 DOCN  M95B0108
 TI    Antiviral effects of plasma and milk proteins: lactoferrin shows potent
       activity against both human immunodeficiency virus and human
       cytomegalovirus replication in vitro.
 DT    9511
 AU    Harmsen MC; Swart PJ; de Bethune MP; Pauwels R; De Clercq E; The TH;
       Meijer DK; Department of Immunology, University of Groningen,
       Netherlands.
 SO    J Infect Dis. 1995 Aug;172(2):380-8. Unique Identifier : AIDSLINE
       MED/95348535
 AB    Native and chemically derivatized proteins purified from serum and milk
       were assayed in vitro to assess their inhibiting capacity on the
       cytopathic effect of human immunodeficiency virus (HIV)-1 and human
       cytomegalovirus (HCMV) on MT4 cells and fibroblasts, respectively. Only
       native and conformationally intact lactoferrin from bovine or human
       milk, colostrum, or serum could completely block HCMV infection (IC50 =
       35-100 micrograms/mL). Moreover, native lactoferrin also inhibited the
       HIV-1-induced cytopathic effect (IC50 = 40 micrograms/mL). When
       negatively charged groups were added to lactoferrin by succinylation,
       there was a 4-fold stronger antiviral effect on HIV-1, but the antiviral
       potency for HCMV infection was mostly decreased. Lactoferrin likely
       exerts its effect at the level of virus adsorption or penetration (or
       both), because after HCMV penetrated fibroblasts, the ongoing infection
       could not be further inhibited.
 DE    Animal  Blood Proteins/*PHARMACOLOGY  Cattle  Cells, Cultured
       Comparative Study  Cytomegalovirus/*DRUG EFFECTS  Cytopathogenic Effect,
       Viral  DNA Replication/DRUG EFFECTS  Embryo  Female  Fibroblasts
       Glycoproteins/PHARMACOLOGY  Human  HIV-1/*DRUG EFFECTS
       Lactoferrin/*PHARMACOLOGY  Lung/DRUG EFFECTS/PATHOLOGY/VIROLOGY  Milk
       Proteins/*PHARMACOLOGY  Pregnancy  Support, Non-U.S. Gov't  Virus
       Inhibitors/*PHARMACOLOGY  Virus Replication/DRUG EFFECTS  JOURNAL
       ARTICLE

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