Document 0114
 DOCN  M95B0114
 TI    Selective early increases of bronchoalveolar CD8+ lymphocytes in a LEW
       rat model of hypersensitivity pneumonitis.
 DT    9511
 AU    Richerson HB; Coon JD; Lubaroff D; Department of Internal Medicine,
       University of Iowa Hospitals,; Iowa City 52242-1081, USA.
 SO    J Allergy Clin Immunol. 1995 Jul;96(1):113-21. Unique Identifier :
       AIDSLINE MED/95348396
 AB    BACKGROUND: The pathogenesis of hypersensitivity pneumonitis (HP)
       involves cell-mediated hypersensitivity; various bronchoalveolar T-cell
       subsets with uncertain roles in disease have been reported and
       implicated in the pathogenesis. OBJECTIVES: Previous studies at 72 hours
       after initial antigen challenges showed proportionate increases in
       T-cell phenotypes. Therefore we tested the hypothesis that early events
       in response to inhaled antigen in a LEW rat model of HP would include a
       disproportionate appearance in bronchoalveolar lavage fluid (BALF) and
       lung parenchyma of a specific T-effector cell responsible for subsequent
       inflammation and that these events could be identified by phenotyping.
       METHODS: We double labeled BALF and parenchymal lung lymphocytes with
       monoclonal antibodies and used flow cytometry to quantitate CD4+ and
       CD8+ phenotypic subsets 4 and 24 hours after inhalation of antigen.
       RESULTS: We found disproportionate increases in BALF CD8+ phenotypes.
       The strongest correlation with pathologic findings was for a putative
       cytotoxic effector (CD8+CD45R-) T lymphocyte. CONCLUSION: Meaningful
       interpretation of lung T-cell phenotype quantitation requires studies of
       kinetics of cellular influxes, timing after antigen challenge, and
       relative comparison with increases in other phenotypes. Any pathogenetic
       role assigned to a phenotype must also await functional studies,
       including cytokine generation and secretion and cell-cell interactions
       in situ.
 DE    Alveolitis, Extrinsic Allergic/*IMMUNOLOGY/*PATHOLOGY  Animal
       Bronchoalveolar Lavage Fluid/*CYTOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY/*PATHOLOGY  Female  Immunophenotyping  Rats
       Rats, Inbred Lew  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).