Document 0129
 DOCN  M95B0129
 TI    Specificity and cross-reactivity of anti-galactocerebroside antibodies.
 DT    9511
 AU    McAlarney T; Ogino M; Apostolski S; Latov N; Department of Neurology,
       College of Physicians and Surgeons of; Columbia University, New York,
       N.Y. 10032, USA.
 SO    Immunol Invest. 1995 May;24(4):595-606. Unique Identifier : AIDSLINE
       MED/95347775
 AB    Anti-galactocerebroside (GalC) antibodies have been reported to inhibit
       myelin formation, cause demyelination, and block HIV-I infection of
       neural cells. We examined the binding of 3 monoclonal and polyclonal
       anti-GalC antibodies to a panel of purified glycolipids by ELISA and by
       an immunospot assay on nitrocellulose blots. All 3 antibodies bound
       strongly to GM1 ganglioside, monogalactosyl diglyceride, and asialo-GM1,
       and 2 of the antibodies bound to GD1b and psychosine. The anti-GalC
       antibodies also bound to 3 glycoprotein bands in human neuroblastoma
       cells on Western blot, and binding to the proteins was abolished by
       pre-treatment with pronase or with periodate which oxidizes the terminal
       carbohydrate residues. These results indicate that anti-GalC antibodies
       cross react with oligosaccharide determinants of other glycolipids and
       glycoproteins, and that these cross-reactivities may be responsible for
       some of the biological effects of the anti-GalC antibodies.
 DE    Animal  Antibody Specificity  Antigenic Determinants/IMMUNOLOGY
       Antigens, Neoplasm/IMMUNOLOGY/ISOLATION & PURIF
       Autoantibodies/IMMUNOLOGY  Carbohydrate Sequence  Comparative Study
       Cross Reactions  Enzyme-Linked Immunosorbent Assay
       Galactosylceramides/*IMMUNOLOGY  Glycolipids/IMMUNOLOGY/ISOLATION &
       PURIF  Glycoproteins/IMMUNOLOGY/ISOLATION & PURIF  Human  IgM/IMMUNOLOGY
       Mice  Molecular Sequence Data  Motor Neuron Disease/IMMUNOLOGY  Neoplasm
       Proteins/IMMUNOLOGY/ISOLATION & PURIF
       Neuroblastoma/CHEMISTRY/IMMUNOLOGY  Rabbits  Sensation
       Disorders/IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).