Document 0139
 DOCN  M95B0139
 TI    Peripheral deletion of autoreactive CD8+ T cells in transgenic mice
       expressing H-2Kb in the liver.
 DT    9511
 AU    Bertolino P; Heath WR; Hardy CL; Morahan G; Miller JF; Walter and Eliza
       Hall Institute of Medical Research, P.O. Royal; Melbourne Hospital,
       Victoria, Australia.
 SO    Eur J Immunol. 1995 Jul;25(7):1932-42. Unique Identifier : AIDSLINE
       MED/95347392
 AB    The response of T cells specific for liver antigens was examined in
       transgenic mice expressing the allogeneic major histocompatibility
       complex class I molecule H-2Kb (Kb) under the control of the sheep
       metallothionein promoter (Met-Kb mice). To follow the fate of
       Kb-specific T cells, and to prevent any aberrant thymic expression of
       the Kb transgene, the mice were thymectomized, lethally irradiated,
       protected with bone marrow cells from transgenic mice expressing in
       their T cells a Kb-specific T cell receptor identifiable by a clonotypic
       antibody, and given syngeneic non-transgenic thymus grafts. Although
       Kb-specific CD8+ T cells were produced in the thymus grafts of these
       manipulated Met-Kb mice, only small numbers of such cells could be
       detected in the spleen and lymph nodes. The livers, however, showed
       signs of damage and were heavily infiltrated by actively dividing CD8+ T
       cells. We provide strong evidence that the hepatocytes, not generally
       regarded as antigen-presenting cells, activated the Kb-specific CD8+ T
       cells and that these disappeared after a vigorous autoimmune response
       that resulted in deletion.
 DE    Animal  Bone Marrow/CYTOLOGY  Cell Survival  Clonal Deletion
       CD8-Positive T-Lymphocytes/*CYTOLOGY  Genes, MHC Class I  H-2
       Antigens/*IMMUNOLOGY  Liver/CYTOLOGY/*IMMUNOLOGY  Lymphocyte
       Transformation  Mice  Mice, Transgenic  Radiation Chimera  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Thymus Gland/CYTOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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