Document 0141
 DOCN  M95B0141
 TI    Comparative analysis of B7-1 and B7-2 expression in Langerhans cells:
       differential regulation by T helper type 1 and T helper type 2
       cytokines.
 DT    9511
 AU    Kawamura T; Furue M; Department of Dermatology, Yamanashi Medical
       University, Japan.
 SO    Eur J Immunol. 1995 Jul;25(7):1913-7. Unique Identifier : AIDSLINE
       MED/95347389
 AB    Epidermal Langerhans cells (LC) are Ia-bearing potent antigen-presenting
       cells (APC) of dendritic cell lineage that play a crucial role in
       primary and secondary T cell-dependent immune responses. LC express
       several costimulatory molecules such as B7, which has been implicated as
       one of the important determinants of professional APC. Recently, B7
       antigens have been shown to include three distinct molecules termed
       B7-1, B7-2, and B7-3, and the expression of B7-1 and B7-2 in LC has been
       already confirmed. However, little is known of the regulation of B7-1
       and B7-2 expression in LC. We demonstrated that LC do not express B7-1
       and B7-2 in situ; however, the expression of both molecules is rapidly
       induced during the first 3 days of culture, and high levels of
       expression are maintained at least until day 6. We show that the
       expression of B7-2 in LC is much higher than that of B7-1 in each
       experiment, and that B7-1 and B7-2 expression is reproducibly augmented
       by interleukin (IL)-4 in a dose-dependent manner; however, IL-2 affected
       expression very little. Finally, B7-1 expression is significantly and
       dose-dependently down-regulated by interferon (IFN)-gamma or IL-10, and
       B7-2 expression is consistently inhibited by IL-10, but not by
       IFN-gamma. The effects of these cytokines are active only in the
       induction phase (during first 3 days of culture) of B7 expression: the
       modulatory effects of cytokines are hardly detected in the plateau phase
       (days 4 to 6 of culture) of B7 expression in LC. These findings suggest
       that B7-1 and B7-2 expression are indeed selectively and differentially
       regulated by these T cell-derived cytokines, and that the cytokines may
       modulate the synthesis of B7 molecules rather than the degradation of
       already-expressed B7 molecules.
 DE    Animal  B-Cell Activation Antigen/*METABOLISM  Cells, Cultured
       Cytokines/*PHARMACOLOGY  Female  In Vitro  Interferon-gamma,
       Recombinant/PHARMACOLOGY  Interleukin-10/PHARMACOLOGY
       Interleukin-2/PHARMACOLOGY  Interleukin-4/PHARMACOLOGY  Langerhans
       Cells/*METABOLISM  Membrane Glycoproteins/*METABOLISM  Mice  Mice,
       Inbred C3H  Support, Non-U.S. Gov't  Th1 Cells/*PHYSIOLOGY  Th2
       Cells/*PHYSIOLOGY  JOURNAL ARTICLE

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