Document 0145
 DOCN  M95B0145
 TI    Tetanus toxoid-specific T cell responses in severe combined
       immunodeficiency (SCID) mice reconstituted with human peripheral blood
       lymphocytes.
 DT    9511
 AU    Somasundaram R; Jacob L; Herlyn D; Wistar Institute, Philadelphia, PA
       19104, USA.
 SO    Clin Exp Immunol. 1995 Jul;101(1):94-9. Unique Identifier : AIDSLINE
       MED/95347089
 AB    SCID mice reconstituted with human peripheral blood lymphocytes (PBL)
       have repeatedly been shown to produce antigen-specific B cell responses.
       We have derived tetanus toxoid (TT)-specific human T cell lines from
       cells of the peritoneal cavity, spleen and lymph nodes of SCID mice
       reconstituted with human PBL and boosted with TT. Establishment of these
       cell lines was dependent on the time interval between reconstitution of
       the mice with human PBL and initiation of lymphocyte cultures in vitro.
       When lymphocytes were collected from the mice 8 weeks after
       reconstitution, human lymphocytes with TT-specific proliferative
       activity in vitro were isolated from the peritoneal cavity and spleen,
       but long-term cell lines could not be established after repeated
       lymphocyte stimulation with TT, IL-2 and autologous Epstein-Barr
       virus-transformed B cells. In contrast, three long-term (> 10 months)
       TT-specific human T cell lines were established from lymphocytes
       collected from two of the eight mice in the group 4 weeks after
       reconstitution. The T cell lines were either CD4+ (two lines derived
       from peritoneal cavity and lymph node, respectively) or CD8+ (one line
       derived from spleen) and all expressed CD3, T cell receptor alpha/beta,
       and human histocompatibility leucocyte class I antigen. The T cell
       lines, however, lacked cytotoxic, helper and suppressor activities.
       Thus, SCID mice can support human T cells that actively migrate to
       various organs and respond to antigenic stimuli both in vivo and in
       vitro, but these T cells lack characteristic functions.
 DE    Animal  Antigens, CD4/BIOSYNTHESIS  Antigens, CD8/BIOSYNTHESIS  Cell
       Line  Human  Hybridomas/IMMUNOLOGY  IgG/BIOSYNTHESIS  Lymphocyte
       Transformation/IMMUNOLOGY  *Lymphocyte Transfusion  Mice  Mice, SCID
       Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/IMMUNOLOGY/METABOLISM/TRANSPLANTATION  Tetanus
       Toxoid/ADMINISTRATION & DOSAGE/*IMMUNOLOGY  Transplantation
       Chimera/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).