Document 0147
 DOCN  M95B0147
 TI    Defects in antigen-driven lymphocyte responses in common variable
       immunodeficiency (CVID) are due to a reduction in the number of
       antigen-specific CD4+ T cells.
 DT    9511
 AU    Funauchi M; Farrant J; Moreno C; Webster AD; Department of Clinical
       Immunology, Royal Free Hospital School of; Medicine, London, UK.
 SO    Clin Exp Immunol. 1995 Jul;101(1):82-8. Unique Identifier : AIDSLINE
       MED/95347087
 AB    T cells from patients with CVID have defects that may relate to the
       failure in vivo of B cell production of antibodies. Antigen-driven
       responses of T cells from CVID patients and normal subjects have been
       assessed by measuring DNA synthesis in vitro. Low density cells enriched
       for antigen-presenting dendritic cells were pulsed with purified protein
       derivative (PPD) and cultured with autologous T cells. Overall, T cells
       from CVID patients showed a significantly low mean response to PPD,
       although non-specific DNA synthesis induced in CVID T cells by IL-2 was
       within the normal range. However, mean PPD-specific T cell responses in
       CVID were not restored by IL-2 irrespective of the presence of
       monocytes. Depletion of CD8+ cells also failed to restore the mean PPD
       response of CVID CD4+ T cells. Limiting dilution analysis showed that in
       CVID there was a reduced frequency of antigen-specific cells within the
       T cell preparations. The mean frequency of the PPD-specific T cells in
       cultures from patients vaccinated with bacille Calmette-Guerin (BCG) was
       reduced to 1 in 109,000 T cells compared with 1 in 18,600 T cells in
       BCG-vaccinated normal donors. These data show that the reduced
       PPD-specific response in CVID is due to a partial peripheral loss of
       antigen-specific cells.
 DE    Adult  Cells, Cultured  Common Variable Immunodeficiency/*IMMUNOLOGY
       CD4-Positive T-Lymphocytes/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Human  IgG/BIOSYNTHESIS  IgM/BIOSYNTHESIS
       Interleukin-2/IMMUNOLOGY  Lymphocyte Transformation/*IMMUNOLOGY
       Mycobacterium bovis/IMMUNOLOGY  Support, Non-U.S. Gov't
       Tuberculin/*IMMUNOLOGY  JOURNAL ARTICLE

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