Document 0157
 DOCN  M95B0157
 TI    Antisense approaches to cancer gene therapy.
 DT    9511
 AU    Mercola D; Cohen JS; San Diego Regional Cancer Center, CA 92121, USA.
 SO    Cancer Gene Ther. 1995 Mar;2(1):47-59. Unique Identifier : AIDSLINE
       MED/95346705
 AB    Recent advances in the use of oligodeoxynucleotide and plasmid-derived
       RNA as antisense agents of special relevance to cancer gene therapy are
       summarized with emphasis on agents and systems which have lead to
       clinical trials and/or regression of established tumors in animal model
       systems. Transformed cell lines bearing plasmids and viruses designed
       for the transcription of antisense RNA have the advantage that they can
       be characterized thoroughly and the effects of antisense RNA on target
       gene expression and phenotype can be studied easily in vivo. Promising
       results make the considerable efforts of applying oligodeoxynucleotides
       in whole animals and in clinical trials more plausible. Conversely,
       oligodeoxynucleotide experiments which yield promising results in tissue
       culture can be generalized to the in vivo setting by development of
       clones of cells bearing plasmid-derived antisense RNA against the same
       target. Several examples of the concordant results for
       oligodeoxynucleotide and plasmid-derived antisense RNA against the same
       target are considered. The importance of examination of antisense
       effects in syngeneic and immunocompetent hosts is illustrated by studies
       of insulin-like growth factor and insulin-like growth factor receptor
       where tumor regression and protection against tumor formation have been
       observed for particular cell types in defined settings.
 DE    Acquired Immunodeficiency Syndrome/GENETICS/THERAPY  Animal  Base
       Sequence  Cell Line  Clinical Trials  Forecasting  Gene Expression  Gene
       Therapy/*METHODS  Genetic Vectors  Growth Substances/GENETICS  Human
       Immunocompetence  Mice  Models, Biological  Molecular Sequence Data
       Neoplasms/GENETICS/*THERAPY  Neoplasms, Experimental/GENETICS/THERAPY
       Oligonucleotides, Antisense/ADMINISTRATION & DOSAGE/GENETICS/
       PHARMACOLOGY/*THERAPEUTIC USE  Oncogenes  Rats  RNA,
       Catalytic/THERAPEUTIC USE  RNA, Messenger/ANTAGONISTS & INHIB  RNA,
       Neoplasm/ANTAGONISTS & INHIB  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  Transcription, Genetic/DRUG EFFECTS  JOURNAL ARTICLE
       REVIEW  REVIEW, ACADEMIC

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).