Document 0165
 DOCN  M95B0165
 TI    Accurate determination of DNA in agarose gels using the novel algorithm
       GelScann(1.0).
 DT    9511
 AU    Metzker ML; Allain KM; Gibbs RA; Department of Molecular and Human
       Genetics, Baylor College of; Medicine, Houston, TX 77030, USA.
 SO    Comput Appl Biosci. 1995 Apr;11(2):187-94. Unique Identifier : AIDSLINE
       MED/95346415
 AB    GelScann(1.0) is a user-friendly program that accurately quantitates DNA
       from CCD imaged agarose gels. The algorithm automatically locates lanes,
       locates bands within a given lane, and quantitates the intensity of each
       band. GelScann (1.0) uses a statistical method to discriminate DNA from
       local background fluorescence. The sensitivity of the LaneFinder and
       BandFinder can be adjusted by the user interacting with GelScann (1.0)'s
       option box. We demonstrate that GelScann(1.0) can accurately determine
       DNA that GelScann(1.0) can accurately determine DNA concentrations for
       routine molecular biology applications and determine the relative
       intensities of PCR amplified fragments for genetic testing.
 DE    *Algorithms  Bacteriophage M13/GENETICS  DNA, Viral/*ANALYSIS
       Electrophoresis, Agar Gel/*METHODS  Female  Heterozygote Detection
       Human  HIV Infections/VIROLOGY  HIV-1/GENETICS  *Image Processing,
       Computer-Assisted  Microcomputers  Muscular Dystrophy/GENETICS
       Polymerase Chain Reaction  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).