Document 0167
 DOCN  M95B0167
 TI    HIV-1 coat protein gp120-induced increases in levels of
       intrasynaptosomal calcium.
 DT    9511
 AU    Nath A; Padua RA; Geiger JD; Department of Medical Microbiology,
       University of Manitoba; Faculty of Medicine, Winnipeg, Canada.
 SO    Brain Res. 1995 Apr 24;678(1-2):200-6. Unique Identifier : AIDSLINE
       MED/95346297
 AB    The effects of human immunodeficiency virus type-1 (HIV-1) coat protein
       gp120 on levels of intrasynaptosomal calcium ([Ca2+]i) were determined
       in rat cortical synaptosomes. gp120 at concentrations of > or = 400 pM,
       significantly (P < 0.05) increased levels of [Ca2+]i. Treatment with 20
       mM KCl, reduced the concentrations of gp120 necessary to produce
       significant (P < 0.001) increases in [Ca2+]i. gp120-evoked increases in
       [Ca2+]i were prevented either by treatment with dantrolene or by removal
       of extracellular calcium with BAPTA. The peak levels of gp120-induced
       increases in [Ca2+]i were not affected by calcium channel blockers
       lanthanum and nicardipine, by glutamate receptor antagonists MK-801 and
       NBQX, or by removal of endogenous glutamate with glutamate
       dehydrogenase. gp120-induced [Ca2+]i increases in presynaptic terminals
       may play a role in HIV-mediated effects in the central nervous system.
 DE    Animal  Calcium/*METABOLISM  Dantrolene/PHARMACOLOGY  Dose-Response
       Relationship, Drug  Fura-2  HIV Envelope Protein gp120/*PHARMACOLOGY
       HIV-1/METABOLISM  Male  Potassium Compounds/PHARMACOLOGY  Rats  Rats,
       Sprague-Dawley  Support, Non-U.S. Gov't  Synaptosomes/*METABOLISM  Time
       Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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