Document 0170
 DOCN  M95B0170
 TI    Encephalitozoon cuniculi isolated from the urine of an AIDS patient,
       which differs from canine and murine isolates.
 DT    9511
 AU    Hollister WS; Canning EU; Colbourn NI; Aarons EJ; Department of Biology,
       Imperial College of Science, Technology &; Medicine, London, United
       Kingdom.
 SO    J Eukaryot Microbiol. 1995 Jul-Aug;42(4):367-72. Unique Identifier :
       AIDSLINE MED/95345842
 AB    A species of Encephalitozoon has been isolated from the urine of a
       patient with the acquired immunodeficiency syndrome and maintained in
       vitro in Madin Darby Canine Kidney cells. When examined by random
       amplified polymorphic DNA polymerase chain reaction the new isolate was
       found to differ from E. hellem and to have amplified products in common
       with murine and canine E. cuniculi. However, it more closely resembled
       the canine than the murine isolate. Sodium dodecylsulphate
       polyacrylamide gel electrophoresis differentiated between all three
       isolates of E. cuniculi, with a band at 42-45 kDa present in the murine
       isolate only, bands at 52 kDa present in the canine and human isolates
       but not the murine, and a single band at 60 kDa (murine) and 65 kDa
       (canine) replaced by two bands at 55 and 70 kDa in the human isolate.
       The 55 kDa and 70 kDa antigens were also revealed as characteristic
       bands of the human isolate by Western blotting. The study has thus
       revealed that the species Encephalitozoon cuniculi is not a homogeneous
       entity.
 DE    Animal  Antigens, Protozoan/ANALYSIS  AIDS-Related Opportunistic
       Infections/PARASITOLOGY/*URINE  Base Sequence  Blotting, Western  Case
       Report  Cell Line  Comparative Study  Dogs/*PARASITOLOGY  DNA Primers
       DNA, Protozoan/ANALYSIS/GENETICS  Electrophoresis, Polyacrylamide Gel
       Encephalitozoon cuniculi/GENETICS/*ISOLATION & PURIF/  ULTRASTRUCTURE
       Encephalitozoonosis/DIAGNOSIS/*ETIOLOGY/URINE  Human  Kidney
       Mice/*PARASITOLOGY  Microscopy, Electron  Molecular Sequence Data
       Polymerase Chain Reaction/*METHODS  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).