Document 0179
 DOCN  M95B0179
 TI    Recovery of human immunodeficiency virus from peritoneal dialysis
       effluent.
 DT    9511
 AU    Scheel PJ Jr; Farzadegan H; Ford D; Malan M; Watson A; Department of
       Medicine, The Johns Hopkins Medical Institutions,; Baltimore, MD, USA.
 SO    J Am Soc Nephrol. 1995 May;5(11):1926-9. Unique Identifier : AIDSLINE
       MED/95345382
 AB    Increasing numbers of HIV-infected patients who have ESRD are being
       treated with continuous ambulatory peritoneal dialysis (CAPD). To
       investigate the potential infectious nature of peritoneal dialysate
       (PD), the peritoneal dialysis effluent was studied in 14 patients on
       CAPD who were known to be HIV antibody positive. Peripheral blood
       mononuclear cells and the sediment of PD were obtained from each patient
       and subjected to a qualitative microculture assay using a coculture of
       patient cells or PD fluid with peripheral blood mononuclear cells from
       non-HIV-infected individuals. Samples from the coculture were collected
       twice weekly for HIV P24 antigen determination as a marker of viral
       replication. PD, white blood cell and red blood cell counts, and
       peripheral blood CD4 lymphocyte counts were also measured. All 14
       patients developed a positive blood culture by Day 3. Twelve of the 14
       patients developed a positive PD fluid culture. The mean CD4 count was
       310 cells/mm3. No patient had clinical or cellular evidence of
       peritonitis at the time of fluid sampling. These data indicate that
       peritoneal dialysis effluent from patients who are HIV antibody positive
       is potentially infectious.
 DE    Adult  CD4 Lymphocyte Count  *Dialysis Solutions  Female  Human  HIV
       Infections/*COMPLICATIONS  HIV-1/*ISOLATION & PURIF  Infection Control
       Kidney Failure, Chronic/*COMPLICATIONS/THERAPY  Male  Middle Age
       *Peritoneal Dialysis, Continuous Ambulatory  JOURNAL ARTICLE

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