Document 0218
 DOCN  M95B0218
 TI    [Heterogeneity of antimicrosomal autoantibodies in chronic hepatitis C
       virus infection and delta hepatitis]
 DT    9511
 AU    Durazzo M; Costa C; Schmidt E; Loges S; Scaglione L; Touscoz AG; Smedile
       A; Manns MP; Rizzetto M; Pagano G; Istituto di Medicina Interna,
       Universita degli Studi di Torino.
 SO    Ann Ital Med Int. 1995 Apr-Jun;10(2):93-7. Unique Identifier : AIDSLINE
       MED/95344920
 AB    Microsomal antigen autoantibodies are typical of type 2 autoimmune
       hepatitis, and a strong association with chronic hepatitis C virus (HCV)
       infection has been reported in certain geographical areas. These
       autoantibodies have been denominated LKM-1 to differentiate them from
       those associated with thienylic acid-induced hepatitis (LKM-2) and from
       those seen in patients with chronic delta hepatitis (LKM-3). To
       investigate the antigenic specificity of autoantibodies associated with
       chronic hepatitis C and delta, we analyzed 52 LKM-1 positive serum
       samples from patients with chronic hepatitis C and 17 LKM-3 positive
       serum samples from patients with chronic delta hepatitis by indirect
       immunofluorescence and Western blotting (immunoblotting). Reactivity of
       subjects with chronic hepatitis C was heterogeneous: only 5 out of 52
       LKM-1 positive patients, tested by Western blot, recognized a single
       protein of 50 kD, previously identified by Manns et al. with an
       immunogenic epitope of cytochrome P450IID6. Thirteen of the 52 patients
       also reacted with a 70 kD microsomal protein, and 12 out of 52 reacted
       only with a 59 kD protein. Twenty-two sera, notwithstanding the high
       titer in immunofluorescence, did not evidence any reactivity when tested
       by Western blot. The same sera tested positive in LKM-1 ELISA when
       solubilized human microsomal proteins were used. Fourteen out of 17
       LKM-3 positive sera from patients with chronic hepatitis delta
       recognized a 55 kD microsomal protein in Western blot; three sera, HCV
       and HIV positive, did not react with any protein by Western blot. None
       of these sera was positive in ELISA LKM-1.(ABSTRACT TRUNCATED AT 250
       WORDS)
 DE    Antigenic Determinants  Autoantibodies/*ANALYSIS  Blotting, Western
       Chronic Disease  Comparative Study  Delta Infection/*IMMUNOLOGY  English
       Abstract  Enzyme-Linked Immunosorbent Assay  Female  Fluorescent
       Antibody Technique  Hepatitis C/*IMMUNOLOGY  Human  Kidney/IMMUNOLOGY
       Male  Microsomes/*IMMUNOLOGY  Microsomes, Liver/IMMUNOLOGY  Population
       JOURNAL ARTICLE

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