Document 0262
 DOCN  M95B0262
 TI    Lymphocytic choriomeningitis virus infection is associated with
       long-standing perturbation of LFA-1 expression on CD8+ T cells.
 DT    9511
 AU    Andersson EC; Christensen JP; Scheynius A; Marker O; Thomsen AR;
       Institute of Medical Microbiology and Immunology, University of;
       Copenhagen, Denmark.
 SO    Scand J Immunol. 1995 Jul;42(1):110-8. Unique Identifier : AIDSLINE
       MED/95357611
 AB    Flow cytometric analysis of splenocytes from mice infected with
       lymphocytic choriomeningitis virus revealed marked and long-standing
       up-regulation of LFA-1 expression on CD8+, but not on CD4+ T cells.
       Appearance of CD8+ T cells with a changed expression of adhesion
       molecules reflected polyclonal activation and expansion which was
       demonstrated not to depend on CD4+ T cells or their products. Cell
       sorting experiments defined virus-specific CTL to be included in this
       population (LFA-1hiMEL-14lo), but since about 80% of splenic CD8+ T
       cells have a changed phenotype, extensive bystander activation must take
       place; this is indicated also by the finding that CD8+LFA-1hi cells
       transiently express several markers of cellular activation, e.g.
       transferrin receptor, IL-2R alpha and beta. Analysis of cells from the
       cerebrospinal fluid of mice infected intracerebrally showed that
       virtually all T cells present belonged to the CD8+LFA-1hi subset and,
       correspondingly, the ligand ICAM-1 was found to be up-regulated on
       endothelial cells in the inflamed meninges. Preincubation of LCMV-primed
       donor splenocytes with anti-LFA-1 markedly inhibited the transfer of
       virus-specific delayed-type hypersensitivity to naive recipients.
       Together, these findings indicate that up-regulation of LFA-1 expression
       is a critical factor involved in directing activated CD8+ T cells to
       sites of viral infection.
 DE    Animal  Cell Adhesion Molecules/BIOSYNTHESIS  Cell Movement/IMMUNOLOGY
       CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY/*METABOLISM  Female  Immunophenotyping
       Immunotherapy, Adoptive  Lymphocyte Function-Associated
       Antigen-1/*BIOSYNTHESIS  Lymphocyte Transformation/IMMUNOLOGY
       Lymphocytic Choriomeningitis/*IMMUNOLOGY  Mice  Mice, Inbred BALB C
       Mice, Inbred C57BL  Spleen/CYTOLOGY  Support, Non-U.S. Gov't
       Up-Regulation (Physiology)/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).