Document 0269
 DOCN  M95B0269
 TI    The use of combined in situ hybridization and immunocytochemistry to
       identify HIV-infected cells in brain tissue.
 DT    9511
 AU    Shapshak P; Yoshioka M; Sun NC; Schiller PC; Department of Psychiatry,
       University of Miami, School of; Medicine, Florida, USA.
 SO    Mod Pathol. 1992 Nov;5(6):649-54. Unique Identifier : AIDSLINE
       MED/95357354
 AB    It is frequently important to identify the types of cells that are
       infected with human immunodeficiency virus type-1 (HIV-1) in sections of
       formalin-fixed paraffin-embedded (FFPE) brain tissue. Currently, both
       immunocytochemical and in situ hybridization methods are used for this
       purpose. Combined in situ hybridization and immunocytochemistry results
       in simultaneous detection of HIV-1 nucleic acids and proteins and allow
       comparison of transcriptional and translational events of cells infected
       with HIV-1 in the same section. In addition, this technique allows
       morphologic and immunologic identification of the cells within which in
       situ hybridization occurs and confirmation of the identity of the cells
       that are not hybridized. Procedures are described for use with FFPE
       brain tissue.
 DE    Brain/*PATHOLOGY/*VIROLOGY  Human  HIV Infections/*PATHOLOGY/*VIROLOGY
       HIV-1/ISOLATION & PURIF  *Immunohistochemistry  *In Situ Hybridization
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).