Document 0271
 DOCN  M95B0271
 TI    Design of four-helix bundle protein as a candidate for HIV vaccine.
 DT    9511
 AU    Eroshkin AM; Karginova EA; Gileva IP; Lomakin AS; Lebedev LR; Kamyinina
       TP; Pereboev AV; Ignat'ev GM; Research Institute of Molecular Biology,
       NPO VECTOR, Koltsovo,; Novosibirsk Region, Russia.
 SO    Protein Eng. 1995 Feb;8(2):167-73. Unique Identifier : AIDSLINE
       MED/95357333
 AB    To be efficient, a synthetic vaccine should contain different T and B
       cell epitopes of human immunodeficiency virus (HIV) antigens, and the B
       epitope regions in the vaccine and in the HIV should be conformationally
       similar. We have suggested previously the construction of vaccines in
       the form of a protein with a predetermined tertiary structure, namely a
       four-alpha-helix bundle. Antigenic determinants of cellular and humoral
       immunity are blocks for the vaccine design. From experimentally studied
       HIV-1 T and B cell epitopes, we constructed a sequence of a four-helix
       protein TBI (T and B cell epitopes containing immunogen). The gene of
       the protein was synthesized and the protein was produced in C600
       Escherichia coli cells under recA promoter from Proteus mirabelis. CD
       spectroscopy of the protein demonstrated that 30% of amino acid residues
       adopt an alpha-helical conformation. Mice immunized with TBI have shown
       both humoral and cellular immune responses to HIV-1. The obtained data
       show that the design of TBI was successful. The synthesized gene
       structure makes possible further reconstruction and improvement of the
       protein vaccine structure.
 DE    Amino Acid Sequence  Animal  Antibody Formation  Antigenic
       Determinants/CHEMISTRY  AIDS Vaccines/*CHEMISTRY/IMMUNOLOGY
       B-Lymphocytes/IMMUNOLOGY  Base Sequence  *Drug Design  Escherichia
       coli/GENETICS  Gene Expression  HIV-1/*IMMUNOLOGY  Immunity, Cellular
       Immunization  Mice  Molecular Sequence Data  Protein Conformation
       *Protein Structure, Secondary  Proteins/*CHEMISTRY/GENETICS/IMMUNOLOGY
       Support, Non-U.S. Gov't  T-Lymphocytes/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).