Document 0282
 DOCN  M95B0282
 TI    Detection of HTLV-I provirus by in situ polymerase chain reaction in
       mononuclear inflammatory cells in skeletal muscle of viral carriers with
       polymyositis.
 DT    9511
 AU    Higuchi I; Hashimoto K; Kashio N; Izumo S; Inose M; Izumi K; Ohkubo R;
       Nakagawa M; Arimura K; Osame M; Third Department of Internal Medicine,
       Kagoshima University,; Japan.
 SO    Muscle Nerve. 1995 Aug;18(8):854-8. Unique Identifier : AIDSLINE
       MED/95356758
 AB    We tested for HTLV-I proviral DNA in skeletal muscle from patients with
       polymyositis infected with HTLV-I using the in situ polymerase chain
       reaction. We found the HTLV-I provirus in some of the CD4-positive cells
       in HTLV-I-positive polymyositis cases but not in HTLV-I-negative
       polymyositis ones. We could not detect HTLV-I within the muscle fibers.
       We suggest that HTLV-I-associated polymyositis is not due to direct,
       persistent infection of the muscle fiber by the virus, but to a
       T-cell-mediated immunological process triggered by the HTLV-I-infected
       cells.
 DE    Adult  Aged  CD4-Positive T-Lymphocytes/*VIROLOGY  DNA, Viral/ANALYSIS
       Female  Human  HTLV-I/GENETICS/*ISOLATION & PURIF  Immunohistochemistry
       Lymph Nodes/CYTOLOGY/VIROLOGY  Male  Middle Age  Muscle,
       Skeletal/*CYTOLOGY/VIROLOGY  Polymerase Chain Reaction/METHODS
       Polymyositis/*VIROLOGY  Proviruses/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).