Document 0296
 DOCN  M95B0296
 TI    Isolation and characterization of an immortal neoplastic cell line (KS
       Y-1) from AIDS-associated Kaposi's sarcoma [see comments]
 DT    9511
 AU    Lunardi-Iskandar Y; Gill P; Lam VH; Zeman RA; Michaels F; Mann DL; Reitz
       MS Jr; Kaplan M; Berneman ZN; Carter D; et al
 SO    J Natl Cancer Inst. 1995 Jul 5;87(13):974-81. Unique Identifier :
       AIDSLINE MED/95356240
 CM    Comment in: J Natl Cancer Inst 1995 Jul 5;87(13):947-9
 AB    BACKGROUND: Acquired immunodeficiency syndrome (AIDS) is associated with
       the occurrence of tumors such as Kaposi's sarcoma (KS) and B-cell
       lymphoma. However, no evidence exists yet that human immunodeficiency
       virus type 1, the causative agent of AIDS, is directly responsible for
       cell transformation. It is also not clear whether KS lesions, which are
       of complex cellularity, contain tumor cells derived from a true
       monoclonal malignancy (originating from a single malignant cell) or
       whether the lesions are just polyclonally hyperplastic in nature
       (containing increased numbers of normal cells). In fact, the presence of
       malignant KS cells has never been unequivocally shown in AIDS-associated
       KS, and previously isolated KS cell cultures were not immortal or
       malignant. PURPOSE: Our purpose was to (a) utilize technology that could
       facilitate isolation and enrichment of tumor cells from AIDS-associated
       KS lesions, (b) establish and characterize an immortalized KS cell line,
       and (c) test the malignant potential of such a cell line in animal
       models. METHODS: Mononuclear cells were isolated from 2.5 L of pleural
       effusion from an AIDS-associated KS patient. T-lymphocytes,
       B-lymphocytes, monocytes/macrophages, and fibroblasts were removed by a
       cytotoxicity method, using monoclonal antibodies specific for cell
       surface markers and baby rabbit complement. KS cells were cultured in
       the absence of exogenous growth factors in an effort to select for
       transformed cells capable of self-sustained growth. The karyotype
       abnormalities were detected by G-banded marker studies, and phenotypic
       markers were determined by indirect immunofluorescence and
       immunocytochemical methods. Beige nude XID and severe combined
       immunodeficient mice were used to evaluate the tumorigenic, angiogenic,
       and metastatic potentials of cells. RESULTS: An immortalized cell line,
       named KS Y-1, was isolated. Its phenotype is similar to that of
       endothelial cells with positive CD34 and CD31 markers. Tetraploid
       chromosomal abnormalities were found in primary fresh KS tissue and in
       vitro passages of KS Y-1 cells. These cells promoted tumorigenesis,
       angiogenesis, and metastasis in immunodeficient mice. Tumors produced at
       the site of injection as well as metastases in the lung, spleen,
       pancreas, gastrointestinal tract, and skin showed a human tetraploid
       karyotype. KS Y-1 cells show high plating efficiency. CONCLUSION: The KS
       Y-1 cell line could be the first evidence of AIDS-associated KS cells
       that may develop clones with an indisputable malignant cell phenotype.
       IMPLICATIONS: KS Y-1 cells in the in vivo mouse model can be used to
       study the effects of therapeutic compounds in advanced KS.
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS  Animal  Disease
       Models, Animal  Human  Karyotyping  Mice  Mice, Inbred Strains  Sarcoma,
       Kaposi's/*ETIOLOGY/GENETICS/IMMUNOLOGY/*PATHOLOGY  Support, U.S. Gov't,
       P.H.S.  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).