Document 0014
 DOCN  M9650014
 TI    The Vpr protein of human immunodeficiency virus type 1 binds to
       nucleocapsid protein p7 in vitro.
 DT    9605
 AU    Li MS; Garcia-Asua G; Bhattacharyya U; Mascagni P; Austen BM; Roberts
       MM; Department of Medical Microbiology, St. George's Hospital Medical;
       School, University of London, U.K.
 SO    Biochem Biophys Res Commun. 1996 Jan 5;218(1):352-5. Unique Identifier :
       AIDSLINE MED/96136326
 AB    The Vpr protein of human immunodeficiency virus type 1 (HIV-1) is
       incorporated into the virion by the Gag polyprotein precursor Pr55gag.
       The importance of the p6gag sequence at the C-terminal end of Pr55gag
       has a crucial role in Vpr incorporation. To identify the Gag sequences
       directly involved in Vpr binding, we compared the Vpr binding affinities
       of the 71 amino acid nucleocapsid protein p7, the C-terminal peptide
       (35-71) p7C and p6gag by affinity chromatography. p7 and p7C have the
       strongest Vpr binding activities compared to p6gag. These results
       suggest that the nucleocapsid protein and its C-terminal domain may be
       important for the incorporation of Vpr into the mature HIV-1 virion and
       the subsequent localisation of viral nucleic acid to the cell nucleus by
       Vpr.
 DE    Blotting, Western  Capsid/CHEMISTRY/ISOLATION & PURIF/*METABOLISM
       Chromatography, Affinity  Electrophoresis, Polyacrylamide Gel  Gene
       Products, gag/ISOLATION & PURIF/METABOLISM  Gene Products,
       vpr/CHEMISTRY/ISOLATION & PURIF/*METABOLISM  Human  HIV-1/*METABOLISM
       Peptide Fragments/CHEMICAL SYNTHESIS/METABOLISM  Protein Binding
       Support, Non-U.S. Gov't  Viral Core Proteins/CHEMISTRY/ISOLATION &
       PURIF/*METABOLISM  Virion/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).