Document 0016
 DOCN  M9650016
 TI    Inhibition of NF-kappa B activation in human T-cell lines by
       anetholdithiolthione.
 DT    9605
 AU    Sen CK; Traber KE; Packer L; Department of Molecular & Cell Biology,
       University of; California-Berkeley 94720-3200, USA.
 SO    Biochem Biophys Res Commun. 1996 Jan 5;218(1):148-53. Unique Identifier
       : AIDSLINE MED/96136291
 AB    Nuclear factor (NF)-kappa B is a redox sensitive cytosolic transcription
       factor. Redox regulation of NF-kappa B has been implicated in the
       activation of the human immuno-deficiency virus (HIV). Therefore,
       inhibition of NF-kappa B activation may be an effective strategy for
       acquired immunodeficiency syndrome therapy. Anetholdithiolthione (ADT,
       5-[p-methoxyphenyl]-3H-1,2-dithiol-3-thione) is an antioxidant which has
       been used to protect against acetaminophen- and CCl4-induced
       hepatotoxicity, lipid peroxidation, radiation injury, and also has been
       used clinically as an anti-choleretic agent. The present study examined
       the effect of ADT pretreatment on NF-kappa B activation in response to a
       variety of stimuli such as H2O2, phorbol myristate acetate (PMA) or
       tumor necrosis factor alpha (TNF alpha). PMA and TNF alpha induced
       activation of (NF)-kappa B in human Jurkat T-cells was partially
       inhibited by ADT (0.1 mM) pretreatment. ADT (0.1 mM) also inhibited H2O2
       induced activation of the transcription factor in the peroxide sensitive
       human Wurzburg T-cells. Furthermore, ADT treated Wurzburg cells had
       significantly higher glutathione levels as compared with untreated
       cells. H2O2 induced lipid peroxidation in Wurzburg cells was remarkably
       inhibited by ADT pretreatment. ADT, a pro-glutathione antioxidant, was
       observed to be capable of modulating NF-kappa B activation.
 DE    Anethole Trithione/*PHARMACOLOGY  Cell Line  Cell Nucleus/METABOLISM
       Human  Hydrogen Peroxide/PHARMACOLOGY  Kinetics  Lymphoma, T-Cell
       NF-kappa B/ANTAGONISTS & INHIB/ISOLATION & PURIF/*METABOLISM
       T-Lymphocytes  Tetradecanoylphorbol Acetate/PHARMACOLOGY  Tumor Cells,
       Cultured  Tumor Necrosis Factor/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).