Document 0030
 DOCN  M9650030
 TI    Uptake of human immunodeficiency virus envelope protein gp120 by human
       trophoblast in culture.
 DT    9605
 AU    Esterman AL; Finlay TH; Lee JD; Dancis J; Department of Pediatrics, New
       York University Medical Center, NY; 10016, USA.
 SO    Am J Obstet Gynecol. 1996 Jan;174(1 Pt 1):49-54. Unique Identifier :
       AIDSLINE MED/96148667
 AB    OBJECTIVE: Our purpose was to determine whether human trophoblast has a
       cell surface CD4 antigen that will bind to gp120, the envelope protein
       of human immunodeficiency virus. STUDY DESIGN: Uptake of iodine
       125-labeled gp120 by trophoblast in culture was measured. Particular
       attention was paid to technical details that may have caused the
       contradictory results reported by previous investigators: the source of
       the recombinant gp120, the method of radioiodination, and the isolation
       procedure of trophoblast to ensure elimination of contaminating cells,
       particularly macrophages. RESULTS: Uptake of transferrin-free iodine
       125-labeled gp120 to trophoblast was unaffected by adding a 200 molar
       excess of gp120, by preincubating gp120 with soluble CD4 to block the
       CD4 binding sites on gp120 and by preincubation of trophoblast with a
       blocking antibody to CD4 (OKT4a). In contrast, uptake of gp120 by
       CD4-positive H9 human lymphocytes was reduced 79% by a 200 molar excess
       of gp120 and > 50% by a CD4-blocking antibody. CONCLUSIONS: Uptake of
       gp120 to trophoblast is by a high capacity, CD4-independent mechanism
       that is probably nonspecific and may be related to the mechanism for
       binding other circulating glycoproteins in maternal blood.
 DE    Animal  Antigens, CD4/METABOLISM  Baculoviridae/GENETICS  CD4-Positive
       T-Lymphocytes/METABOLISM  CHO Cells  Electrophoresis, Polyacrylamide Gel
       Female  Hamsters  Human  HIV Envelope Protein gp120/*METABOLISM  *HIV-1
       Iodine Radioisotopes  Pregnancy  Recombinant Proteins/METABOLISM
       Support, U.S. Gov't, P.H.S.  Tissue Culture
       Trophoblast/IMMUNOLOGY/*METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).