Document 0039
 DOCN  M9650039
 TI    Development and application of an antibody ELISA for the marker protein
       of ovine pulmonary carcinoma.
 DT    9605
 AU    Kwang J; Keen J; Rosati S; Tolari F; USDA, ARS, US Meat Animal Research
       Center, Clay Center, NE 68933,; USA.
 SO    Vet Immunol Immunopathol. 1995 Aug;47(3-4):323-31. Unique Identifier :
       AIDSLINE MED/96016705
 AB    Ovine pulmonary carcinoma (OPC) is a contagious pulmonary neoplasia with
       a suspected retroviral etiology. The major core protein (P27) of the
       putative OPC virus cross-reacts with antibodies to P27 of the
       Mason-Pfizer monkey virus (MPMV), a type-D retrovirus. This serological
       reactivity serves as the only accepted biological marker for OPC. In
       order to make a useful reagent for the detection of the OPC marker for
       serodiagnosis and epidemiological studies, the MPMV-P27 coding region
       was cloned and expressed in Escherichia coli. Gel purified recombinant
       MPMV-P27 protein was used to develop an immunoassay. This recombinant
       enzyme-linked immunosorbent assay (ELISA) was then used to screen 223
       sera from US sheep and 176 sera from Italian sheep. In this study, we
       found: (1) a high prevalence of infection with the putative OPC
       retrovirus in sheep with chronic pneumonia; (2) a subclinical infection
       with OPC virus may be more common in US sheep than indicated by the rare
       recorded occurrence of pulmonary carcinoma; (3) an apparent association
       between ovine lentivirus (OLV) and OPC infection.
 DE    Adenocarcinoma, Bronchiolo-Alveolar/DIAGNOSIS/*VETERINARY/  VIROLOGY
       Animal  Antibodies, Viral/ANALYSIS  Base Sequence  Blotting, Western
       DNA Primers/CHEMISTRY  Enzyme-Linked Immunosorbent
       Assay/METHODS/*VETERINARY  Escherichia coli/GENETICS  Gene Expression
       Regulation, Viral  Lung Neoplasms/DIAGNOSIS/*VETERINARY/VIROLOGY  Macaca
       Mason-Pfizer Monkey Virus/GENETICS/IMMUNOLOGY  Molecular Sequence Data
       Prevalence  Recombinant Proteins/IMMUNOLOGY  Retroviridae
       Infections/DIAGNOSIS/*VETERINARY/VIROLOGY  Sensitivity and Specificity
       Sheep  Sheep Diseases/*DIAGNOSIS/VIROLOGY  Tumor Markers, Biological
       Tumor Virus Infections/DIAGNOSIS/*VETERINARY/VIROLOGY  Viral Core
       Proteins/GENETICS/*IMMUNOLOGY  Visna-Maedi Virus/GENETICS/IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).