Document 0071
 DOCN  M9650071
 TI    The Gag-Myb-Ets fusion oncogene alters the apoptotic response and growth
       factor dependence of interleukin-3 dependent murine cells.
 DT    9605
 AU    Athanasiou M; Mavrothalassitis GJ; Yuan CC; Blair DG; Laboratory of
       Cellular Biochemistry, SAIC Frederick, Maryland,; USA.
 SO    Oncogene. 1996 Jan 18;12(2):337-44. Unique Identifier : AIDSLINE
       MED/96152899
 AB    Expression of the avian E26-derived Gag-Myb-Ets fusion oncogene in
       interleukin-3(IL3)-dependent murine hematopoietic cell lines results in
       a pattern of cell line dependent changes in growth factor-induced
       proliferation and apoptosis. A drug-selectable retrovirus expressing
       p135Gag-Myb-Ets induced an erythropoietin(Epo)-responsive phenotype in
       the cell lines FDC-P2, BaF3 and 32Dc123. Gag-Myb-Ets expression alone
       did not increase expression of GATA-1 or the Epo receptor(EpoR) in the
       presence of IL3, and infected cell lines express increased GATA-1 and
       EpoR only when IL3 was replaced by Epo in the culture media. Indicative
       of Epo-induced erythroid differentiation, these cells also began to
       express beta-globin after 3-5 days growth in Epo. Unlike control cells,
       infected FDC-P2 cells failed to undergo programmed cell death
       (apoptosis) when transferred from IL3- to Epo-containing media, although
       a fraction of the cells failed to proliferate following the media shift.
       Three other IL3-dependent cell lines showed no changes in growth
       behavior when induced to express the fusion oncogene. Our data shows
       that Gag-Myb-Ets can have different affects on growth factor pathways
       depending on the cell background, suggesting a model in which the
       p135gag-myb-ets fusion oncogene promotes these different responses
       through its affect on apoptosis.
 DE    Animal  *Apoptosis  Base Sequence  Cell Line  Cloning, Molecular
       Erythropoietin/PHARMACOLOGY  Gene Products, gag/*GENETICS
       Interleukin-3/*PHARMACOLOGY  Mice  Molecular Sequence Data  *Oncogenes
       Proto-Oncogene Proteins/*GENETICS  Transcription Factors/*GENETICS  3T3
       Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).